Platelets and Megakaryocytes

by Gibbins, Jonathan M.; Mahaut-Smith, Martyn P.

• ISBN13: 9781588291011
• ISBN10: 1588291014
• Format: Hardcover
• Copyright: 2004-07-01
• Publisher: Humana Pr Inc

Summary

For both volumes: A comprehensive collection of cutting-edge methods for the molecular and cellular analysis of platelets and megakaryocytes. Volume 1 details basic and advanced techniques for analyzing platelet and megakaryocyte function. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. The techniques for study of megakaryocyte function allow the measurement of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. Volume 2 offers techniques for studying many aspects of signaling in platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts, and the measurement of intracellular calcium levels. There are also techniques for study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. This volume also describes the application of molecular and post-genomic methods for the study of platelets and megakaryocytes. All protocols follow the successful Methods in Molecular Biology'„¢ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The two volumes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Table of Contents

Functional Assays
Preface	v
Contents of Volume II	xiii
Contributors	xv
Color Plates	xix
PART I. PLATELET FUNCTIONAL ASSAYS
1 Effects of Anticoagulants Used During Blood Collection on Human Platelet Function
Jane A. May and Stanley Heptinstall	3	(10)
2 Preparation of Washed Platelet Suspensions From Human and Rodent Blood
Jean-Pierre Cazenave, Philippe Ohlmann, Dominique Cassel, Anita Eckly, Beatrice Hechler, and Christian Gachet	13	(16)
3 Platelet Counting
Paul Harrison, Carol Briggs, and Samuel J. Machin	29	(18)
4 Electron Microscopy Methods for Studying Platelet Structure and Function
James G. White	47	(18)
5 Platelet Aggregation: Turbidimetric Measurements
Gavin E. Jarvis	65	(12)
6 Platelet Aggregation in Whole Blood: Impedance and Particle Counting Methods
Gavin E. Jarvis	77	(12)
7 Secretion From Dense Granules: Luminescence Method for Adenine Nucleotides
M. Fred Heath	89	(6)
8 Platelet Dense-Granule Secretion: The [³H]-5-HT Secretion Assay
David Crosby and Alastair W. Poole	95	(2)
9 Analysis of Releasable Nucleotides of Platelets
Joachim Jankowski, Lars Henning, and Hartmut Schluter	97	(12)
10 Studies of Secretion Using Permeabilized Platelets
Tara W. Rutledge and Sidney W. Whiteheart	109	(12)
11 Measurement of Platelet Arachidonic Acid Metabolism
Richard W. Farndale, Philip G. Hargreaves, Joanna L. Dietrich, and Rosemary J. Keogh	121	(14)
12 Measurement of the Platelet Procoagulant Response
Johan W.M. Heemskerk, Paul Cornfurius, Marion A.H. Feijge, and Edouard M. Bevers	135	(10)
13 Platelet Adhesion Assays Performed Under Static Conditions
Joanne M. Stevens	145	(8)
14 Platelet Adhesion Assays Under Flow Using Matrix Protein-Coupled Adhesion Columns
Renata Polanowska-Grabowska and Adrian R.L. Gear	153	(12)
15 Techniques to Examine Platelet Adhesive Interactions Under Flow
Suhasini Kulkarni, Warwick S. Nesbitt, Sacha M. Dopheide, Sascha C. Hughan, Ian S. Harper, and Shaun P. Jackson	165	(22)
16 In Vivo Models of Platelet Function and Thrombosis: Study of Real-Time Thrombus Formation
Shahrokh Falati, Peter L. Gross, Glenn Merrill-Skoloff, Derek Sim, Robert Flaumenhaft, Alessandro Cell, Barbara C. Furie, and Bruce Furie	187	(12)
17 Adhesion Between Platelets and Leukocytes or Endothelial Cells
Gerard B. Nash	199	(16)
18 In Vitro Measurement of High-Shear Platelet Adhesion and Aggregation by the PFA-100®
Paul Harrison	215	(10)
19 Flow-Cytometric Analysis of Platelet-Membrane Glycoprotein Expression and Platelet Activation
Alison H. Goodall and Jackie Appleby	225	(30)
20 Flow-Cytometric Analysis of Mouse Platelet Function
Bernhard Nieswandt, Valerie Schulte, and Wolfgang Bergmeier	255	(14)
21 Platelet Microparticles
Shosaku Nomura and Shirou Fukuhara	269	(12)
PART II. MEGAKARYOCYTE FUNCTIONAL ASSAYS
22 Isolation of Primary Megakaryocytes and Studies of Proplatelet Formation
Robert M. Leven	281	(12)
23 Isolation and Culture of Megakaryocyte Precursors
Najet Debili, Fawzia Louache, and William Vainchenker	293	(16)
24 Assays of Megakaryocyte Development: Surface Antigen Expression, Ploidy, and Size
Anthony Mathur, Ying Hong, Guosu Wang, and Jorge D. Erusalimsky	309	(14)
25 Assays of Megakaryocyte Development: Cytoplasm, Storage Granules, and Demarcation Membranes
Arnaud Drouin, Gulie Alimardani, and Elisabeth M. Cramer	323	(24)
26 Thrombopoietin Bioassay
Warren S. Alexander and Craig Hyland	347	(14)
27 Culture of Megakaryocytic Cell Lines: Uses and Limitations
Norio Komatsu	361	(14)
Index	375