Prostate Cancermethods and Protocols

by Russell, Pamela J.; Jackson, Paul; Kingsley, Elizabeth A.

• ISBN13: 9780896039780
• ISBN10: 0896039781
• Format: Hardcover
• Copyright: 2003-04-01
• Publisher: Humana Pr Inc

Summary

A collection of basic and advanced molecular methods that reveal those markers essential for more accurate diagnoses of specific diseases, and in developing new treatment strategies. The techniques range from in vitro methods to in vivo models of prostate cancer, and include new methods for the accurate diagnosis of prostate cancer, proteome and microarray analyses, and new strategies for the treatment of refractory disease. Each method is described in step-by-step detail to ensure successful results and avoid failure.

Table of Contents

Preface	v
Contributors	ix
Epidemiological Investigation of Prostate Cancer	1	(20)
Graham G. Giles
Human Prostate Cancer Cell Lines	21	(20)
Pamela J. Russell
Elizabeth A. Kingsley
Growth of Prostatic Epithelial and Stromal Cells In Vitro	41	(18)
Donna M. Peehl
Prostate Epithelial Stem Cell Isolation and Culture	59	(10)
David L. Hudson
John R. W. Masters
Generation of Immortal Human Prostate Cell Lines for the Study of Prostate Cancer	69	(10)
Johng S. Rhim
Spheroids of Prostate Tumor Cell Lines	79	(10)
George Sgouros
Wei-Hong Yang
Richard Enmon
Animal Models of Prostate Cancer	89	(24)
Pamela J. Russell
Dale J. Voeks
Transgenic Mouse Models for Prostate Cancer: Identification of an Androgen-Dependent Promoter and Creation and Characterization of the Long Probasin Promoter-Large T Antigen (LPB-Tag) Model	113	(36)
Susan Kasper
William Tu
Richard L. Roberts
Scott B. Shappell
In Vivo Models of Human Prostate Cancer Bone Metastasis	149	(14)
Julie M. Brown
Effects of Fixation on Tissues	163	(18)
Elin Mortensen
Julie M. Brown
Background, Methods, and Protocols for the Histopathological Diagnosis of Prostate Carcinoma	181	(18)
Warick Delprado
Realizing the Potential of Ejaculate/Seminal Fluid in Detecting and Predicting Natural History	199	(20)
R. A. Gardiner
Michelle Burger
Judith A. Clements
Martin F. Lavin
Bisulfite Methylation Analysis of Tumor Suppressor Genes in Prostate Cancer from Fresh and Archival Tissue Samples	219	(22)
Susan J. Clark
Douglas S. Millar
Peter Molloy
Production and Characterization of Antipeptide Kallikrein 4 Antibodies: Use of Computer Modeling to Design Peptides Specific to Kallikrein 4	241	(14)
Tracey J. Harvey
Ying Dong
Loan Bui
Russell Jarrott
Terry Walsh
Judith A. Clements
The Androgen Receptor CAG Repeat and Prostate Cancer Risk	255	(12)
Peter E. Clark
Ryan A. Irvine
Gerhard A. Coetzee
Studies on Androgen Receptor Mutations and Amplification in Human Prostate Cancer	267	(10)
Zoran Culig
Alfred Hobisch
Martin Erdel
Georg Bartsch
Helmut Klocker
Proteomics in the Analysis of Prostate Cancer	277	(22)
Soren Naaby-Hansen
Kohji Nagano
Piers Gaffney
John R. W. Masters
Rainer Cramer
Application of Gene Microarrays in the Study of Prostate Cancer	299	(22)
Colleen C. Nelson
Douglas Hoffart
Martin E. Gleave
Paul S. Rennie
Enhancer Trap Method Using a Green Fluorescent Protein Reporter Plasmid for Cloning Tissue-Specific Enhancers Active in Prostate Cells	321	(12)
Fujiko Watt
Peter Molloy
Targeted Alpha Therapy of Prostate Cancer	333	(26)
Barry J. Allen
Yong Li
Syed M. A. Rizvi
Pamela J. Russell
Phenotypic and Functional Differences of Dendritic Cells Generated Under Different In Vitro Conditions	359	(18)
Stephanie E. B. McArdle
Selman A. Ali
Geng Li
Shahid Mian
Robert C. Rees
Flavonoid Compounds in the Prevention and Treatment of Prostate Cancer	377	(18)
Graham E. Kelly
Alan J. Husband
Index	395