Protein Engineering Handbook

by Editor: Stefan Lutz (Emory University, Atlanta, USA); Editor: Uwe Theo Bornscheuer (Greifswald University, Germany)

• ISBN13: 9783527318506
• ISBN10: 352731850X
• Edition: Revised
• Format: Hardcover
• Copyright: 2009-01-01
• Publisher: Vch Verlagsgesellschaft Mbh

Summary

Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas.

Author Biography

Stefan Lutz holds a B. S. degree from the Zurich University of Applied Sciences (Switzerland), and a M.S. degree from the University of Teesside (UK). He then obtained a Ph.D. from the University of Florida and spent three years as a Postdoc with Stephen Benkovic at Pennsylvania State University under a fellowship of the Swiss National Science Foundation. Since 2002 he has been a Chemistry professor at Emory University in Atlanta, Georgia (USA). The research in the Lutz laboratory focuses on the structure-function relationship of proteins through combinatorial protein engineering.

Uwe Bornscheuer studied Chemistry at the University of Hannover (Germany), where he obtained a Ph. D. at the Institute of Technical Chemistry. He then spent a postdoctoral year at the University of Nagoya, Japan, before returning to Germany to join the Institute of Technical Biochemistry at the University of Stuttgart. Since 1999 he has been Professor for Biotechnology and Enzyme Catalysis at the University of Greifswald. His main research interest is the application of engineered enzymes in the synthesis of optically active compounds and in lipid modification.

Table of Contents

Guidelines for the Functional Analysis of Engineered and Mutant Enzymes Engineering Enantioselectivity in Enzyme-Catalyzed Reactions

Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identification and Manipulation of Catalytically

Promiscuous Enzymes Phi-Value Analysis of Protein Folding Transition States Protein

Folding and Solubility: Pathways and High-Throughput Assays Protein Dynamics and the Evolution of Novel Protein Function Gaining

Insight into Enzyme Function through Correlation with Protein Motions Structural Frameworks Suitable for Engineering

Microbes and Enzymes: Recent Trends and New Directions to Expand Protein Space Inteins in Protein Engineering

From Prospecting to Product ? Industrial Metagenomics Is Coming of Age Computational

Protein Design Assessing and Exploiting the Persistence of Substrate Ambiguity in Modern Protein Catalysts Designing Programmable

Protein Switches The Cyclization of Peptides and Proteins with Inteins

A Method for Rapid Directed Evolution Evolution of Enantioselective Bacillus subtilis Lipase

Circular Permutation of Proteins Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): A Versatile Tool for Generating

Targeted Libraries Protein Engineering by Structure-Guided SCHEMA Recombination Chimeragenesis in Protein Engineering Protein

Generation Using a Reconstituted System Equipping in vivo Selection Systems with Tunable Stringency Protein Engineering by Phage Display Screening Methodologies for Glycosidic

Bond Formation Yeast Surface Display in Protein Engineering and Analysis In Vitro Compartmentalization (IVC) and Other High-Throughput Screens of Enzyme Libraries Colorimetric and Fluorescence-Based

Screening Confocal and Conventional Fluorescence-Based High Throughput Screening in Protein Engineering 30 Alteration of Substrate Specifi city and Stereoselectivity of Lipases and Esterases

Altering Enzyme Substrate and Cofactor Specifi city via Protein Engineering Protein Engineering of Modular Polyketide Synthases

Cyanophycin Synthetases Biosynthetic Pathway Engineering Strategies Natural Polyester-Related Proteins: Structure, Function, Evolution and Engineering

Bioengineering of Sequence-Repetitive Polypeptides: Synthetic Routes to Protein-Based

Materials of Novel Structure and Function Silk Proteins ?

Biomaterials and Bioengineering

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