Proteomics in Functional Genomics

by Jolles, Pierre; Jornvall, Hans

• ISBN13: 9783764358853
• ISBN10: 3764358858
• Format: Hardcover
• Copyright: 2000-05-01
• Publisher: Birkhauser

Summary

A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...

Table of Contents

List of Contributors	vii
Protein structure analysis of today: Proteomics in functional genomics	xi
Hans Jornvall
Pierre Jolles
Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis	1	(28)
Ettore Appella
David Arnott
Kazuyasu Sakaguchi
Peter J. Wirth
Techniques for sample preparation including methods for concentrating peptide samples	29	(14)
Kris Gevaert
Tony Houthaeve
Joel Vandekerckhove
Sample preparation by SDS/PAGE and in-gel digestion	43	(12)
Ulf Hellman
High-resolution two-dimensional gel electrophoresis and protein identification using western blotting and ECL detection	55	(14)
Julio E. Celis
Pavel Gromov
Nanospray mass spectrometry in protein and peptide chemistry	69	(12)
William J. Griffiths
MALDI-TOF mass spectrometry in protein chemistry	81	(18)
Peter Roepstorff
The chemistry of protein sequence analysis	99	(20)
John E. Shively
The alkylated thiohydantoin method for C-terminal sequence analysis	119	(14)
David R. Dupont
MeriLisa Bozzini
Victoria L. Boyd
Ladder sequencing	133	(12)
Tomas Bergman
New approaches for innovations in sensitive Edman sequence analysis by design of a wafer-based chip sequencer	145	(14)
Christian Wurzel und
Brigitte Wittmann-Liebold
Analysis of glycoprotein-derived glycopeptides	159	(28)
Gerrit J. Gerwig
Johannes F. G. Vliegenthart
Lipopeptide preparation and analysis	187	(12)
Jan Johansson
Margareta Stark
Magnus Gustafsson
Yuqin Wang
Shahparak Zaltash
Phosphopeptide analysis	199	(16)
Manfredo Quadroni
Peter James
Bioinformatics in protein analysis	215	(18)
Bengt Persson
Subject index	233