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9780121012908

Pulsed Field Gel Electrophoresis : A Practical Guide

by ; ; ;
  • ISBN13:

    9780121012908

  • ISBN10:

    0121012905

  • Edition: Spiral
  • Format: Paperback
  • Copyright: 1993-02-01
  • Publisher: Academic Pr

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Supplemental Materials

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Summary

Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels.

Table of Contents

Introduction to Pulsed Field Gel Electrophoresis
PFGE Instrumentation
Preparation of DNA for Pulsed Field Analysis
Pouring Gels and Handling Samples
Restriction Enzyme Digestion of Large DNA Fragments in Agarose
Switch Intervals and Resolution in Pulsed Field Gels
Field Inversion Gel Electrophoresis (FIGE)
Variables Which Affect Pulsed Field Gels
Southern Blot Analysis of Pulsed Field Gels
Special Applications
Trouble Shooting
Bibliography of Pulsed Field Gel References
Appendices
Chapter References
Table of Contents provided by Publisher. All Rights Reserved.

Supplemental Materials

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The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

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