Recombinant Proteins from Plants

by Cunningham, Charles, Ph.D.; Porter, Andrew J. R.

• ISBN13: 9780896033900
• ISBN10: 0896033902
• Format: Hardcover
• Copyright: 1998-01-01
• Publisher: Humana Pr Inc

Summary

This authoritative collection of basic and advanced protocols permits biotechnologists to use plants rather than single cell fermentation systems for expressing recombinant proteins. The protocols provide clear step-by-step instructions for the cloning, expression, and analysis of a wide variety of recombinant proteins in plants, and for the cloning of genes into a number of different plant species. The book also describes promising experimental techniques that will become increasingly important in the future, and includes techniques for the characterization of medically important proteins expressed in plants.

Table of Contents

Preface	v	(4)
Contributors	ix
1 Rice Transformation by Agrobacterium Infection	1	(10)
Xiongying Cheng
Ravinder K. Sardana
Illimar Altosaar
2 Barley as a Producer of Heterologous Protein	11	(16)
Leena Mannonen
Kristian Aspegren
Anneli Ritala
Hanna Simola
Teemu H. Teeri
3 Introducing and Expressing Genes in Legumes	27	(12)
Adrian D. Bavage
Mark P. Robbins
Leif Skot
K. Judith Webb
4 Use of the GUS Reporter Gene	39	(10)
Joy Wilkinson
Keith Lindsey
5 Expression of Recombinant Proteinase Inhibitors in Plants	49	(16)
Dominique Michaud
Thierry C. Vrain
6 Detection of Recombinant Viral Coat Protein in Transgenic Plants	65	(12)
Herta Steinkellner
Irina Korschhineck
7 Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants	77	(12)
Ravinder K. Sardana
Peter R. Ganz
Anil Dudani
Eilleen S. Tackaberry
Xiongying Cheng
Illimar Altosaar
8 Tobamovirus Vectors for Expression of Recombinant Genes in Plants	89	(14)
Thomas H. Turpen
Stephen J. Reinl
9 Single-Chain Fv Antibodies Expressed in Plants	103	(26)
Udo Conrad
Ulrike Fiedler
Olga Artsaenko
Julian Phillips
10 Characterization and Applications of Plant-Derived Recombinant Antibodies	129	(14)
Rainer Fischer
Jurgen Drossard
Yu-Cai Liao
Stefan Schillberg
11 Production of Recombinant Antibodies in Plant Suspension Cultures	143	(12)
Jurgen Drossard
Yu-Cai Liao
Rainer Fischer
12 Production of Foreign Proteins in Tobacco Cell Suspension Culture	155	(10)
Veronique Gomord
Anne-Catherine Fitchette-Laine
Lise-Anne Denmat
Dominique Michaud
Loic Faye
13 Transient Gene Expression in Plant Protoplasts	165	(12)
Stefan Schillberg
Sabine Zimmermann
Dirk Prufer
Detlef Schuman
Rainer Fischer
14 Stability of Recombinant Proteins in Plants	177	(12)
Dominique Michaud
Thierry C. Vrain
Veronique Gomord
Loic Faye
15 Trafficking and Stability of Heterologous Proteins in Transgenic Plants	189	(14)
Johnathan A. Napier
Gaelle Richard
Peter R. Shewry
16 Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein	203	(26)
Myriam De Neve
Helena Van Houdt
Anne-Marie Bruyns
Marc Van Montagu
Ann Depicker
17 Manipulation of Photosynthetic Metabolism	229	(22)
Martin A.J. Parry
Steven P. Colliver
Pippa J. Madgwick
Matthew J. Paul
18 Qualification of Heterologous Protein Levels in Transgenic Plants by ELISA	251	(20)
Anne-Marie Bruyns
Myriam De Neve
Geert De Jaeger
Chris De Wilde
Pierre Rouze
Ann Depicker
19 Analysis of N- and O- Glycosylation of Plant Proteins	291	(14)
Anne-Catherine Fitchette-Laine
Lise-Anne Denmat
Patrice Lerouge
Loic Faye
N.P. Eswara Reddy
M. Jacobs
Index	305