Regulation of Gene Expression by Small Rnas

by Gaur; Rajesh K.

• ISBN13: 9780849391699
• ISBN10: 0849391695
• Edition: 1st
• Format: Hardcover
• Copyright: 2009-04-27
• Publisher: CRC Press

Summary

Recent research demonstrates that defects in the processing mechanisms of small non-coding RNAs are linked to various human diseases. This text explores the latest research on microRNAs and their functional roles in cells and organisms. Written by international experts, discussion will include the identification, characterization, and structure-function analysis of microRNAs; the study of microRNAs in development, cancer, and viruses; and the applications of microRNAs with respect to gene regulation, functional genomics and therapeutics. This book is a valuable reference for established researchers and is also suitable for postdoctoral and clinical fellows, and graduate students.

Table of Contents

Preface	p. ix
Acknowledgments	p. xi
About the Editors	p. xiii
Contributors	p. xv
MicroMining: Computational Approaches to microRNA Discovery	p. 1
Animal microRNA Gene Prediction	p. 21
A Suite of Resources for the Study of microRNA Ontology and Function	p. 45
Regulation of Translation and mRNA Stability by Hfq-Binding Small RNAs in Escherichia coli	p. 57
Mechanisms by which microRNAs Regulate Gene Expression in Animal Cells	p. 77
The microRNAs of Caenorhabditis elegans	p. 89
Isolation and Characterization of Small RNAs in Caenorhabditis elegans	p. 101
MicroRNA Tales in Fly Development	p. 123
RNA Interference and microRNAs in Zebra Fish	p. 149
Biogenesis and Function of Plant microRNAs	p. 173
Endogenous Small RNA Pathways in Arabidopsis	p. 197
How to Assay microRNA Expression: A Technology Guide	p. 215
Methods to Quantify microRNA Gene Expression	p. 241
Regulation of Alternative Splicing by microRNAs	p. 259
Recent Progress in Polymerase II-Mediated Intronic microRNA Expression Systems	p. 275
MicroRNA-Based RNA Polymerase II Expression Vectors for RNA Interference in Mammalian Cells	p. 301
Transgenic RNAi: A Fast and Low-Cost Approach to Reverse Genetics in Mammals	p. 317
Symphony of AIDS: A microRNA-Based Therapy	p. 333
MicroRNAs and Cancer: Connecting the Dots	p. 351
Mammalian Transcriptional Gene Silencing by Small RNAs	p. 393
Regulation of Gene Expression by RNA-Mediated Transcriptional Gene Silencing	p. 405
Index	p. 419
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