Single-Molecule Biophysics Experiment and Theory, Volume 146

by Komatsuzaki, Tamiki; Kawakami, Masaru; Takahashi, Satoshi; Yang, Haw; Silbey, Robert J.; Rice, Stuart A.; Dinner, Aaron R.

• ISBN13: 9781118057803
• ISBN10: 1118057805
• Edition: 1st
• Format: Hardcover
• Copyright: 2011-11-22
• Publisher: Wiley

Summary

Single-molecule experiments have provided fresh perspectives to questions such as how proteins fold to specific conformations from highly heterogeneous structures, how signal transductions take place on the molecular level, and how proteins behave in membranes and live cells. This book focuses mainly on three experimental approaches: single-molecule fluorescence based mainly on fluorescence resonance energy transfer (FRET), atomic force microscopy (AFM) and diffracted X-ray tracking (DXT), and their applications to important biological phenomena. In addition, discussion is provided on the theoretical progress in the single-molecule data analyses. For students and professionals in chemical physics and physical chemistry from academia, government, and industries.

Author Biography

Tamiki Komatsuzaki is a Professor in the Molecule and Life Nonlinear Sciences Laboratory, Research Institute for Electronic Science at Hokkaido University in Japan. His research interests include origin of selectivity and stochasticity of reactions, single-molecule biophysics, as well as protein landscape and complexity in kinetics and dynamics. Masaru Kawakami is an Associate Professor at the Japan Advanced Institute of Science and Technology (JAIST) School of Material Science. He specializes in biophysics, single-molecule measurement, protein folding, and structural biology, and his research focuses on single-molecule dynamics of biomolecules. Satoshi Takahashi is a Professor at the Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Japan. His research focuses on protein folding and functional dynamics. Haw Yang is an Associate Professor in the Department of Chemistry at Princeton University. His current research focuses on high-resolution quantitative single-molecule protein dynamics and real-time 3D single-particle tracking spectroscopy. Robert J. Silbey is the Class of 1942 Professor of Chemistry at MIT. His research involves theoretical studies of single-molecule spectroscopy at low temperatures, energy and electron transfer and relaxation in molecular aggregates, the optical and electronic properties of conjugated oligomers and polymers, and the transport of charge in organic systems. Stuart A. Rice received his master's and doctorate from Harvard University and was a junior fellow at Harvard for two years before joining the faculty of the University of Chicago in 1957, where he is currently the Frank P. Hixon Distinguished Service Professor Emeritus. Aaron R. Dinner received his bachelor's degree and doctorate from Harvard University, after which he conducted postdoctoral research at the University of Oxford and the University of California, Berkeley. He joined the faculty at the University of Chicago in 2003.

Table of Contents

Preface	p. xiii
Developments on Single-Molecule Experiments
Staring at a Protein: Ensemble and Single-Molecule Investigations on Protein-Folding Dynamics	p. 3
Single-Molecule FRET of Protein-Folding Dynamics	p. 23
Quantitative Analysis of Single-Molecule FRET Signals and its Application to Telomere DNA	p. 49
Force to Unbind Ligand-Receptor Complexes and the Internal Rigidity of Globular Proteins Probed by Single-Molecule Force Spectroscopy	p. 71
Recent Advances in Single-Molecule Biophysics with the Use of Atomic Force Microscopy	p. 89
Dynamical Single-Molecule Observations of Membrane Protein Using High-Energy Probes	p. 133
Single-Molecular Gating Dynamics for the KcsA Potassium Channel	p. 147
Static and Dynamic Disorder in in vitro Reconstituted Receptor-Adaptor Interaction	p. 195
Developments on Single-Molecule Theories and Analyses
Change-Point Localization and Wavelet Spectral Analysis of Single-Molecule Time Series	p. 219
Theory of Single-Molecule FRET Efficiency Histograms	p. 245
Multidimensional Energy Landscapes in Single-Molecule Biophysics	p. 299
Generalized Michaelis-Menten Equation for Conformation Modulated Monomeric Enzymes	p. 329
Making it Possible: Constructing a Reliable Mechanism from a Finite Trajectory	p. 367
Free Energy Landscapes of Proteins: Insights from Mechanical Probes	p. 395
Mechanochemical Coupling Revealed by the Fluctuation Analysis of Different Biomolecular Motors	p. 419
Author Index	p. 437
Subject Index	p. 467
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