Stroke Genomics

by Read, Simon J.; Virley, David

• ISBN13: 9781588293336
• ISBN10: 1588293335
• Format: Hardcover
• Copyright: 2004-10-01
• Publisher: Humana Pr Inc

Summary

Leading experts explore the pragmatic application of the tools of genomic and molecular biology research to the treatment of stroke. Providing the reader with cutting-edge reviews of clinical and preclinical genomics, the authors relate changes in gene expression to physiological endpoints-such as functional imaging paradigms-to produce a more holistic approach in which molecular biology goes hand-in-hand with stroke pathophysiology. Topics of special interest include stem cell transplantation, gene therapy, clinical gene/gene interaction studies, and cytokine drug discovery. Readily reproducible techniques are provided, along with ancillary methods (imaging, animal models, human genetic study design, and transgenic animal production) that can be effectively applied to gene discovery. The book shows how to successfully design, execute, and interpret preclinical and clinical molecular biological studies of stroke, work that will greatly advance its understanding, as well as rapid development of the next generation of stroke therapeutics.

Table of Contents

Preface	v
Contributors	ix
PART I. INTRODUCTION
1 Introduction to Stroke Genomics
Simon J. Read and Frank C. Barone	3	(16)
PART II. PRECLINICAL MODELS AND GENE MANIPULATION
2 Choice, Methodology, and Characterization of Focal Ischemic Stroke Models: The Search for Clinical Relevance
David Virley	19	(30)
3 Mutant Animal Models of Stroke and Gene Expression: The Stroke-Prone Spontaneously Hypertensive Rat
Hilary V.O. Carswell, Martin W. McBride, Delyth Graham, Anna F. Dominiczak, and I. Mhairi Macrae	49	(26)
4 Gene Therapy in Neurological Disease
Midori A. Yenari and Robert M. Sapolsky	75	(14)
5 Stem Cell Transplantation After Middle Cerebral Artery Occlusion
Paul Stroemer and Helen Hodges	89	(16)
6 Endogenous Brain Protection: Models, Gene Expression, and Mechanisms
Frank C. Barone	105	(80)
7 Production of Transgenic and Mutant Mouse Models
Alex J. Harper	185	(18)
8 3-Nitropropionic Acid Model of Metabolic Stress: Assessment by Magnetic Resonance Imaging
Toby John Roberts	203	(20)
PART III. CLINICAL PARADIGMS
9 Practicalities of Genetic Studies in Human Stroke
Ahamad Hassan and Hugh S. Markus	223	(18)
10 Evaluation of the Interactions of Common Genetic Mutations in Stroke
Zoltán Szolnoki	241	(12)
PART IV. ASSESSING DIFFERENTIAL EXPRESSION
11 Technologies of Disease-Related Gene Discovery Using Preclinical Models of Stroke
Xinkang Wang	253	(12)
12 Quantitative Analysis of Gene Transcription in Stroke Models Using Real-Time RT-PCR
David C. Harrison and Brian C. Bond	265	(20)
13 Effective Analysis of Genomic Data
Paul R. Nelson, Andrew B. Goulter, and Richard J. Davis	285	(28)
14 Bioinformatic Approaches to Assigning Protein Function From Novel Sequence Data
David Michalovich and Richard Fagan	313	(20)
15 Pragmatic Target Discovery From Novel Gene to Functionally Defined Drug Target: The Interleukin-1 Story
Stuart McRae Allan	333	(14)
Index	347