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9780199634941

Subcellular Fractionation A Practical Approach

by ;
  • ISBN13:

    9780199634941

  • ISBN10:

    0199634947

  • Format: Paperback
  • Copyright: 1997-03-27
  • Publisher: IRL Press

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Summary

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes and other organelles. Basic separation techniques needed in all cell biology laboratories are given and presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles. Subcellular Fractionation contains expert advice and detailed methodology, and will be invaluable to researchers in this important area.

Table of Contents

Homogenization of tissues and cells
Isolation of subcellular fragments
Isolation and characterization of nuclei and nuclear subfractions
Subcellular fractionation of mitochondria
Isolation and characterization of peroxisomes
Lysosomes and endocytosis
The membranes of the secretory and exocytic pathways
Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells
Ribosomes and polysomes
Electron microscopy of organelles
Table of Contents provided by Publisher. All Rights Reserved.

Supplemental Materials

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