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Techniques for Microbiology A Student Handbook

by
Edition:
1st
ISBN13:

9780132240116

ISBN10:
0132240114
Format:
Paperback
Pub. Date:
10/24/2006
Publisher(s):
Benjamin Cummings
List Price: $40.60

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Summary

This vivid, full-color laboratory techniques handbook is an instructive, concise, graphical presentation of the skills and techniques required in an introductory microbiology lab. Clear visual instructions enable readers to carry out fundamental manipulations and procedures effectively and safely.Demonstrates those techniques that will be used frequently for studying microbes in the laboratory. Has a safety section and frequent safety cautions throughout. Has a convenient, portable 6" x 9" trim size, a spiral binding and soft cover, making it ideal for use on the lab bench surface. It is priced inexpensively so that it will be suitable as a supplement to an in-house or commercial manual.Companion to any introductory laboratory whether for biology majors or allied health majors.

Table of Contents

Preface ix
About the Author xi
Unit I Safety First! 1(5)
Safety in the Microbiology Laboratory
1(4)
Universal Human Blood and Body Fluid Precautions
5(1)
Unit II Culturing Bacteria and Aseptic Techniques 6(68)
Preparing Culture Media
6(11)
Sterilization
17(6)
The Autoclave
17(1)
Dry Heat
18(1)
Filtration
19(4)
Adjusting the Gas Burner
23(4)
Using an Electrical Incinerator (Bacti-Cinercitor)
27(1)
Aseptic Transfer of Bacteria
28(14)
Preparation of the Work Area
28(1)
Transferring from a Broth Culture into a Broth Tube
29(3)
Transferring from a Broth Culture onto an Agar Slant
32(3)
Transferring from an Agar Plate to an Agar Slant
35(2)
Transferring Bacteria while Holding Multiple Tubes
37(3)
Inoculating an Agar Deep Using the Stab Technique
40(2)
Isolation of Bacteria
42(18)
Preparing a Streak Plate
42(6)
Preparing a Pour Plate
48(8)
Preparing a Spread Plate
56(4)
Characteristic Features of Bacterial Growth in Culture
60(3)
Maintenance and Storage of Stock Cultures
63(7)
Short-term Storage of Stock Cultures
63(1)
Short- and Long-term Storage
63(2)
Growing a Fresh Culture from Frozen Stock
65(5)
Culturing Anaerobic Bacteria
70(4)
Unit III Visualizing Bacteria 74(47)
Effective Use and Responsible Care of the Light Microscope
74(10)
Viewing Live Microorganisms
84(3)
Preparing a Hanging-drop Slide
84(3)
Staining Bacteria
87(30)
Preparing a Bacterial Smear
87(5)
Preparing a Simple Stain
92(2)
Preparing a Gram Stain
94(5)
Preparing an Acid-fast Stain
99(6)
Preparing a Negative Stain
105(4)
Preparing a Capsule Stain
109(3)
Preparing an Endospore Stain
112(5)
Measuring Microscopic Cells
117(4)
Unit IV Enzyme-based Tests for the Identification of Bacteria 121(57)
Hydrolytic (Digestive) Enzymes
121(10)
Starch Hydrolysis Test
121(2)
Casein Hydrolysis Test
123(2)
Gelatin Hydrolysis Test
125(2)
Fat (Triglyceride) Hydrolysis Test
127(2)
DNA Hydrolysis Test
129(2)
Utilization of Carbohydrates
131(11)
Fermentation of Carbohydrates (Durham Tube)
131(2)
Methyl Red Test (Mixed Fermentation)
133(2)
Voges-Proskauer Test (Butanediol Fermentation)
135(2)
Citrate Utilization Test
137(2)
Oxidation—fermentation (OF) Glucose Test
139(3)
Degradation of Amino Acids
142(8)
Indole (Tryptophan Degradation) Test
142(2)
Hydrogen Sulfide (H2S) Production Test
144(2)
Phenylalanine Deamination Test
146(2)
Amino Acid Decarboxylase Test
148(2)
Respiration-Tests
150(7)
Catalase Test
150(2)
Oxidase Test
152(2)
Nitrate Reduction Test
154(3)
Miscellaneous Tests
157(11)
Triple Sugar Iron (TSI) Agar Test
157(2)
Urea Hydrolysis Test
159(2)
Litmus Milk Reactions
161(3)
Motility Testing
164(2)
Coagulase (Tube) Test
166(2)
Selective and/or Differential Media
Blood Agar Plate
168(2)
Eosin Methylene Blue (EMB) Agar
170(2)
Mannitol Salt Agar
172(2)
MacConkey Agar
174(2)
Phenylethyl Alcohol (PEA) Agar
176(2)
Unit V Counting Microbes 178(28)
Direct Counting with the Petroff-Hausser Chamber
178(5)
Preparing a Standard Plate Count of viable Bacteria
183(8)
Using Turbidimetry to Estimate Cell Density
191(7)
Determining Bacteriophage Titer by a Plaque Assay
198(8)
Unit VI Measuring the Effectiveness of Antibacterial Chemicals 206(12)
Testing Antibacterial Medicines: Kirby-Bauer Technigue
206(7)
Testing Antiseptics and Disinfectants: Disk Diffusion Technique
213(5)
Appendix: Stains and Reagents 218(5)
Index 223


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