Telomerase Inhibition

by Andrews, Lucy; Tollefsbol, Trygve O.

• ISBN13: 9781588296832
• ISBN10: 1588296830
• Edition: 1st
• Format: Hardcover
• Copyright: 2007-12-30
• Publisher: Humana Pr Inc

Summary

This volume presents a compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition. The techniques described provide the researcher with a diverse and comprehensive set of tools for the study of telomerase inhibition.

Table of Contents

Preface	p. v
Contributors	p. ix
Methods of Telomerase Inhibition	p. 1
Telomerase Inhibition by Synthetic Nucleic Acids and Chemosensitization in Human Bladder Cancer Cell Lines	p. 9
hTERT Knockdown in Human Embryonic Kidney Cells Using Double-Stranded RNA	p. 23
RNA Interference Using a Plasmid Construct Expressing Short-Hairpin RNA	p. 31
Retrovirus-Mediated RNA Interference	p. 39
Telomerase Inhibition and Telomere Targeting in Hematopoietic Cancer Cell Lines with Small Non-Nucleosidic Synthetic Compounds (BIBR1532)	p. 47
Uses of Telomerase Peptides in Anti-Tumor Immune Therapy	p. 61
Establishing Cell-Based Reporter Systems for the Analysis of hTERT Expression	p. 87
Telomerase RNA Inhibition Using Antisense Oligonucleotide Against Human Telomerase RNA Linked to a 2',5'-Oligoadenylate	p. 97
Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA Interference	p. 113
Evaluation of Tankyrase Inhibition in Whole Cells	p. 133
Inhibition of Telomerase by Targeting MAP Kinase Signaling	p. 147
Screening of Telomerase Inhibitors	p. 167
Telomerase Inhibition Combined with Other Chemotherapeutic Reagents to Enhance Anti-Cancer Effect	p. 181
Index	p. 191
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