Telomeres and Telomerase

by Double, John A.; Thompson, Michael J.

• ISBN13: 9780896036574
• ISBN10: 089603657X
• Format: Hardcover
• Copyright: 2002-03-01
• Publisher: Humana Pr Inc

Summary

John A. Double and Michael J. Thompson have collected a critically important series of novel and essential techniques for studying telomeres and telomerase. These readily reproducible methods provide cutting-edge tools to identify, measure, and analyze telomeres, to determine telomerase expression at the RNA level, to determine telomerase activity, and to detect potential modifiers of this activity. The techniques for assaying telomerase activity range from standard radiological TRAP assays to nonradioactive methods, from non PCR-based methods to techniques using real-time PCR. Telomeres and Telomerase: Methods and Protocols provides the core array of productive techniques needed today to develop telomerase inhibitors or diagnostic/prognostic telomerase markers.

Table of Contents

Preface	v
Contributors	ix
Introduction to Telomeres and Telomerase	1	(12)
Michael C. Bibby
Detection of Chromosome Ends by Telomere Fish	13	(20)
Harry Scherthan
Telomere Length Distribution: Digital Image Processing and Statistical Analysis	33	(32)
Jean-Patrick Pommier
Laure Sabatier
Analysis of Telomerase RNA Gene Expression by In Situ Hybridization	65	(18)
W. Nicol Keith
Joseph Sarvesvaran
Martin Downey
Relative Gene Expression in Normal and Tumor Tissue by Quantitative RT-PCR	83	(16)
Dennis S. Salonga
Kathleen D. Danenberg
Jean Grem
Ji Min Park
Peter V. Danenberg
Quantitative Detection of Telomerase Components by Real-Time, Online RT-PCR Analysis with the LightCycler	99	(10)
Thomas Emrich
Sheng-Yung Chang
Gerlinde Karl
Birgit Panzinger
Chris Santini
Standard TRAP Assay	109	(16)
Angelika M. Burger
Stretch PCR Assay	125	(12)
Jun-ichi Nakayama
Fuyuki Ishikawa
Fluorescent Detection of Telomerase Activity	137	(10)
Wade K. Aldous
Amber J. Marean
Mary J. DeHart
Katherine H. Moore
Nonradioactive Detection of Telomerase Activity Using a PCR-ELISA-Based Telomeric Repeat Amplification Protocol	147	(12)
Thomas Emrich
Gerlinde Karl
In Situ TRAP Assay Detection of Telomerase Activity in Cytological Preparations	159	(6)
Kazuma Ohyashiki
Junko H. Ohyashiki
Biotinylated Primer for Detecting Telomerase Activity Without Amplification	165	(8)
Daekyu Sun
Whole-Cell and Microcell Fusion for the Identification of Natural Regulators of Telomerase	173	(24)
Henriette Gourdeau
Marsha D. Speevak
Lucie Jette
Mario Chevrette
Screening with COMPARE Analysis for Telomerase Inhibitors	197	(12)
Imad Naasani
Takao Yamori
Takashi Tsuruo
Telomerase as a Therapeutic Target: Therapeutic Potential of Telomerase Inhibitors	209	(8)
John A. Double
Index	217