The Ubiquitous Roles of Cytochrome P450 Proteins

by Sigel, Astrid; Sigel, Helmut; Sigel, Roland K. O.

• ISBN13: 9780470016725
• ISBN10: 0470016728
• Edition: 1st
• Format: Hardcover
• Copyright: 2007-04-30
• Publisher: WILEY

Summary

Helmut Sigel, Astrid Sigel and Roland K.O. Sigel, in close cooperation with John Wiley & Sons launch a new Series "Metal Ions in Life Sciences".There exists a whole range of books on Cytochromes P450, but none with the focus of this volume. This new volume in the Series concentrates on current hot topics in the area and tries to work out the underlying common developments.As a result the reader will find a systematic account of new results in this exciting research area. The table of contents gives an idea on the wide span of chapters, starting with overviews and the presentation of specific systems, and ending with chapters on carbon-carbon bond cleavage by P450 sytems, drug metabolism as catalyzed by P450 systems, decomposition of xenobiotics by P450 enzymes and design and engineering of new P450 systems.

Author Biography

Astrid Sigel has studied languages and was an editor of the Metal Ions in Biological Systems series (until Volume 44) and also of the Handbooks on Toxicity of Inorganic Compounds (1988), on Metals in Clinical and Analytical Chemistry (1994; both with H.G. Seiler), and on Metalloproteins (2001; with Ivano Bertini) (Dekker, New York).

Helmut Sigel is Emeritus Professor (2003) of Inorganic Chemistry at the University of Basel, Switzerland, and a previous editor of the MIBS series until Volume 44. He serves on various editorial and advisory boards, published over 300 articles on metal ion complexes of nucleotides, coenzymes, and other ligands of biological relevance, and lectured worldwide. He was named Protagonist in Chemistry (2002) by ICA (issue 339); among further honors are the P. Ray Award (Indian Chemical Society, of which he is also an Honorary Fellow), the Werner Award (Swiss Chemical Society), a Doctor of Science honoris causa degree (Kalyani University, India), appointments as Visiting Professor (e.g., Austria, China, UK) and Endowed Lectureships.

Roland K.O. Sigel is Assistant Professor (2003) of Inorganic Chemistry at the University of Zürich, Switzerland, endowed with a Förderungsprofessur of the Swiss National Science Foundation. He received his doctoral degree summa cum laude (1999) from the University of Dortmund, Germany, working with Bernhard Lippert; thereafter he spent nearly three years at Columbia University, New York, USA, in the group of Anna Marie Pyle (now Yale University); during the six years abroad he received several fellowships from various sources. His research focuses on the structural and catalytic role of metal ions in ribozymes, especially group II introns, and on related topics. He was also an editor of Volumes 43 and 44 of the MIBS series.

Table of Contents

Historical Development And Perspectives Of The Series

Preface To Volume 3

Contributors To Volume 3

Titles Of Volumes 1-44 In The Metal Ions In Biological Systems Series

Contents Of Volumes In The Metal Ions In Life Sciences Series

Diversities And Similarities In P450 Systems: An Introduction

Oxygenases: Mediators of Biochemical Diversity

P450 Superfamily: Diversity at the Sequence Level

Diversity of P450 Structures: Folds and Conformations for Functions

Diversity in P450 Mechanisms

Diversity in Regulation Across the Superfamily

Diversity in the Evolution of Common Metabolic Functions

Summary and Outlook

Acknowledgments

Abbreviations

References

Structural And Functional Mimics Of Cytochromes P450

Introduction

Iron Porphyrins Carrying a Thiolate or Modified Thiolate Ligand

Structurally Remote P450 Mimics

Concluding Remarks

Acknowledgments

Abbreviations

References

Structures Of P450 Proteins And Their Molecular Phylogeny

Introduction

P450 Evolution

P450 Families and Subfamilies

P450 Structures

Variation in P450 Function and Fold

Archaeon P450s

Summary and Conclusions

Acknowledgments

Abbreviations

References

Aquatic P450 Species

Introduction: 'P450s Under the Surface'

Diversity of Aquatic Species

P450 Activities in Aquatic Invertebrates

Aquatic P450 Gene Families Identified

How Can We Use Information About P450s in Aquatic Species?

Conclusions and Outlook

Acknowledgments

Abbreviations

References

The Electrochemistry Of Cytochrome P450

Introduction

Redox Titration (Potentiometric Equilibrium) Measurements

Voltammetric (Dynamic) Measurements

Conclusions

Acknowledgments

Abbreviations

References

P450 Electron Transfer Reactions

Introduction

Catalytic Cycles

Electron Tunneling Wires

Concluding Remarks

Acknowledgments

Abbreviations

References

Leakage In Cytochrome P450 Reactions In Relation To Protein Structural Properties

Introduction

Protein Structural Parameters

The Reaction Cycle of Cytochrome P450

Protein Structural Parameters and Extent of Competitive Reactions

Concluding Remarks

Acknowledgments

Abbreviations

References

Cytochromes P450 - Structural Basis For Binding And Catalysis

Introduction

Ligand Binding: Substrate Recognition and Access to the Distal Pocket

Architecture of the Active Site of CYP101

The Distal Acid-Alcohol Pair

Experimental Characterization of Reaction Intermediates. Radiolysis as a Tool to Study Redox Reactions

Crystal Structures of Oxy-Ferrous Complexes

Mechanism: Summary, Conclusions, Speculations

Acknowledgments

Abbreviations

References

Beyond Heme-Thiolate Interactions: Roles Of The Secondary Coordination Sphere In Cytochrome P450 Systems

Overview of Cytochrome P450 Active Site Structure

Secondary Coordination Sphere on the Proximal Side

Secondary Coordination Sphere on the Distal Side

Summary and Outlook

Acknowledgments

Abbreviations

References

Interactions Of Cytochrome P450 With Nitric Oxide And Related Ligands

Introduction: Interactions of Ligands and Substrates with P450 Enzymes: General Features

Nitric Oxide and Its Interactions with P450S

Interactions of Imidazoles and Substituted Imidazoles with P450S

Other Ligands and Inhibitors of P450 Function

Conclusions and Future Prospects

Acknowledgments

Abbreviations

References

Cytochrome P450-Catalyzed Hydroxylations And Epoxidations

Introduction

The Cytochrome P450 Enzymes

Three-Dimensional Structures of the Active Sites of Cytoch

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