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9781588295620

Quantum Dots

by ;
  • ISBN13:

    9781588295620

  • ISBN10:

    1588295621

  • Format: Hardcover
  • Copyright: 2007-02-03
  • Publisher: Humana Pr Inc

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Supplemental Materials

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Summary

Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.

Table of Contents

Prefacep. v
Contributorsp. ix
Imaging of Fixed Cells and Tissues
Immunofluorescent Labeling of Proteins in Cultured Cells With Quantum Dot Secondary Antibody Conjugatesp. 3
Immunohistochemical Detection With Quantum Dotsp. 11
Quantum Dots for Multicolor Tumor Pathology and Multispectral Imagingp. 29
Light and Electron Microscopic Localization of Multiple Proteins Using Quantum Dotsp. 43
Human Metaphase Chromosome FISH Using Quantum Dot Conjugatesp. 55
Imaging of Live Cells
Biotin-Ligand Complexes With Streptavidin Quantum Dots for In Vivo Cell-Labeling of Membrane Receptorsp. 69
Single Quantum Dot Tracking of Membrane Receptorsp. 81
Optical Monitoring of Single Cells Using Quantum Dotsp. 93
Peptide-Mediated Intracellular Delivery of Quantum Dotsp. 105
Multiple Cell Lines Using Quantum Dotsp. 113
Measuring Cell Motility Using Quantum Dot Probesp. 125
Imaging of Live Animals
Quantum Dots for In Vivo Molecular and Cellular Imagingp. 135
Sentinel Lymph Node Mapping With Type-II Quantum Dotsp. 147
Macrophage-Mediated Colocalization of Quantum Dots in Experimental Gliomap. 161
Flow Cytometry Applications
Application of Quantum Dots to Multicolor Flow Cytometryp. 175
Quantum Dots in Flow Cytometryp. 185
Biochemical Applications
Luminescent Biocompatible Quantum Dots: A Tool for Immunosorbent Assay Designp. 207
Fluorescence-Based Analysis of Cellular Protein Lysate Arrays Using Quantum Dotsp. 229
Application of Quantum Dots to Multicolor Microarray Experiments: Four-Color Genotypingp. 239
Indexp. 253
Table of Contents provided by Ingram. All Rights Reserved.

Supplemental Materials

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