Scientists working or planning to work in the field of cardiovascular research will welcome Practical Methods in Cardiovascular Research as the reference book they have long been waiting for. Not only general aspects of cardiovascular research are well presented, but also detailed descriptions of methods and protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Practical Methods in Cardiovascular Research is therefore a long awaited "must have" for anybody with an interest in cardiovascular research.

In-Vivo Techniques
Models of Cardiovascular Diseases
Anaesthesia (Inhalative or i.v. Narcosis) of Laboratory Animals	p. 5
Extracorporeal Circulation	p. 26
Open-chest Models of Acute Myocardial Ischemia and Reperfusion	p. 37
Models of Chronic Ischemia and Infarction	p. 58
Models on Left Ventricular Hypertrophy (LVH)	p. 72
Experimental Models of Heart Failure	p. 83
In-Vivo Models of Arrhythmias: a Canine Model of Sudden Cardiac Death	p. 111
In-Vivo Models of Atrial Fibrillation	p. 129
In-Vitro Techniques
Isolated Organs
The Langendorff Heart	p. 155
The Working Heart	p. 173
Isolated Papillary Muscles	p. 190
Isolated Vessels	p. 198
Electrophysiological Techniques
Optical Techniques for the Recording of Action Potentials	p. 215
Recording Action Potentials Using Voltage-Sensitive Dyes	p. 233
Epicardial Mapping in Isolated Hearts - Use in Safety Pharmacology, Analysis of Torsade de Pointes Arrhythmia and Ischemia-Reperfusion-Related Arrhythmia	p. 256
Voltage-Clamp and Patch-Clamp Techniques	p. 272
L-Type Calcium Channel Recording	p. 324
Recording Cardiac Potassium Currents with the Whole-Cell Voltage Clamp Technique	p. 355
Recording the Pacemaker Current If	p. 381
Recording Gap Junction Currents	p. 397
Recording Monophasic Action Potentials	p. 417
Heart on a Chip - Extracellular Multielectrode Recordings from Cardiac Myocytes in Vitro	p. 432
Histological Techniques
Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Research	p. 457
Classical Histological Staining Procedures in Cardiovascular Research	p. 485
Practical Use of in situ Hybridisation and RT in situ PCR in Cardiovascular Research	p. 500
Practical Use of Transmission Electron Microscopy for Analysis of Structures and Molecules in Cardiovascular Research	p. 525
DAF Technique for Real-Time NO Imaging in the Human Myocardium	p. 546
Cell Culture Techniques
Isolation and Culture of Adult Ventricular Cardiomyocytes	p. 557
Culture of Neonatal Cardiomyocytes	p. 568
Culture of Embryoid Bodies	p. 577
Culturing and Differentiation of Embryonic and Adult Stem Cells for Heart Research and Transplantation Therapy	p. 592
Preparation of Endothelial Cells from Micro- and Macrovascular Origin	p. 610
In Vitro Cultivation of Vascular Smooth Muscle Cells	p. 630
Engineering Heart Tissue for In Vitro and In Vivo Studies	p. 640
Biochemical and Analytical Techniques
High-Performance Liquid Chromatography (HPLC)	p. 661
Protein Biochemistry	p. 686
Receptor and Binding Studies	p. 723
Recording and Analysing Concentration-Response Curves	p. 784
Measurement of NO and Endothelial Function in Cardiovascular Research	p. 799
Biomechanical Stimulation of Vascular Cells In Vitro	p. 813
Measurement of Function and Regulation of Adrenergic Receptors	p. 829
Measurement of Function and Regulation of Muscarinic Acetylcholine Receptors	p. 848
Cytometry in Cardiovascular Research	p. 863
Molecular Biology in Cardiovascular Research
Molecular Biology Techniques
Real-Time PCR Analysis of Cardiac Samples	p. 891
Use of cDNA Arrays to Explore Gene Expression in Genetically Manipulated Mice and Cell Lines	p. 907
Using Antibody Arrays to Detect Protein-Protein Interactions	p. 916
Surface Plasmon Resonance as a Method to Study the Kinetics and Amplitude of Protein-Protein Binding	p. 936
How to Solve a Protein Structure by Nuclear Magnetic Resonance - The Connexin43 Carboxyl Terminal Domain	p. 948
Managing a Transgenic Mouse Colony: A Guide for the Novice	p. 959
Appendix
Colour Plates	p. 979
Index	p. 997
Table of Contents provided by Ingram. All Rights Reserved.

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Practical Methods in Cardiovascular Research

9783540407638

3540407634

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