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Preface | p. v |
Contributors | p. ix |
Knock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways | p. 1 |
Functional Dissection of Mitotic Regulators Through Gene Targeting in Human Somatic Cells | p. 21 |
RNAi in Drosophila S2 Cells as a Tool for Studying Cell Cycle Progression | p. 39 |
Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast | p. 63 |
Hydrodynamic Analysis of Human Kinetochore Complexes During Mitosis | p. 81 |
Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells | p. 99 |
Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines | p. 113 |
Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos | p. 135 |
Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells | p. 145 |
Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells | p. 165 |
Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin Under the Native Promoter | p. 185 |
Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression | p. 205 |
Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts | p. 221 |
Live Cell Imaging of Kinetochore Capture by Microtubules in Budding Yeast | p. 233 |
The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery | p. 243 |
Measuring Proteolysis in Mitosis | p. 259 |
An In Vitro Assay for Cdc20-Dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast | p. 271 |
In Vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg Extracts | p. 287 |
Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation | p. 301 |
Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive (Pseudosubstrate) Inhibitors | p. 313 |
Index | p. 331 |
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