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Introduction
Lab Safety
Background
Procedure
Experiment 1: Safety Features and Hazards
-Part 1: Safety Features
-Part 2: Hazards
-Part 3: Leaving the Lab
Experiment 2: Dressing for the Lab
-Part 1: Dress Code
-Part 2: Personal Protective Equipment (PPE)
Introduction to Lab Simulations
Introduction to the Virtual Laboratory
Touring the Virtual Laboratory
Experiment 1: Measure a Change in Temperature Following a Chemical Reaction Between HCl and NaOH
Experiment 2: Investigate the Relationship between the Volume and Pressure of a Gas
Experiment 3: Observing a Reaction Between HCl and Zinc
Scientific Method
Background
Procedure
Experiment 1: Find the Best Mouse Diet for Weight Gain
Experiment 2: Replicate the Experiment
Basic Microscopy
Background
Procedure
Experiment 1: Visualizing Cells Using a Microscope
-Part 1: Visualizing Amoeba Cells
-Part 2: Visualizing Spirogyra Cells
-Part 3: Visualizing Cardiac Muscle Cells
-Part 4: Visualizing Bacterial Cells
Experiment 2: Estimating Size
-Part 1: Calculating Total Magnification
-Part 2: Calibration 1
-Part 3: Calibration 2
-Part 4: Estimating the Size of an Onion Epidermis Cell
-Part 5: Estimating the Width of a Blood Cell (Neutrophil)
-Part 6: Estimating the Width of a Blood Vessel
Microbiology
Aseptic Technique
Background
Procedure
Experiment 1: Pouring an Agar Plate
-Part 1: Introduction
-Part 2: Removing Agar
-Part 3: Pouring Agar
-Part 4: Summary
Experiment 2: Making a Streak Plate Using the Aseptic Technique
Cultivation of Bacteria
Background
Procedure
Experiment 1: Bacteria in the Environment
Experiment 2: Isolation of Bacteria
-Part 1: Streak Plate Method of Isolation
-Part 2: Spread Plate Method of Isolation
Staining
Background
Procedure
Experiment 1: Gram Staining
Part 1: Heat-Fixation of the S. aureus Bacterial Slide
Part 2: Gram Staining of S. aureus
Part 3: Identifying S. aureus with the Microscope
Part 4: Repeating the Gram Staining Protocol with E. coli
Experiment 2: Acid-Fast Staining
-Part 1: Heat-Fixation of the E. coli and M. phlei Bacterial Slides
-Part 2: Acid-Fast Staining of E. coli and M. phlei
Experiment 3: Capsule Staining of K. pneumoniae and S. pneumoniae
Enumeration of Bacteria
Background
Procedure
Experiment 1: Spread Plating of Bacterial Serial Dilutions
Experiment 2: Counting Plate CFUs
Experiment 3: Calculating Sample CFU Density
Motility and Amino Acid Hydrolysis
Background
Procedure
Experiment 1: Motility
Experiment 2: Amino Acid Hydrolysis
Blood
Background
Procedure
Experiment 1: Normal Peripheral Blood
Experiment 2: Diseased Peripheral Blood
Unknown Identification
Background
Procedure
Experiment 1: Gram Stain
-Part 1: Heat-Fixation of the Bacterial Slide
-Part 2: Staining
-Part 3: Identifying the Bacteria with the Microscope
Experiment 2: Starch Hydrolysis Test
Experiment 3: Citrate Test
Experiment 4: Anaerobic Jar
Control of Bacterial Growth
Background
Procedure
Experiment 1: UV Radiation
Experiment 2: Chemical Agent Effects on E. coli
Experiment 3: Pressurized Steam, High-Temperature Sterilization Using an Autoclave
Bacterial Transformation
Background
Procedure
Experiment 1: Bacterial Transformation with an Ampicillin-Resistant Plasmid
Experiment 2: Determining Transformation Success Using Selective Media
Extreme Bacteria
Background
Procedure
Experiment 1: Effect of Temperature on Microbial Growth
Experiment 2: Effect of pH on Microbial Growth
Experiment 3: Effect of Osmotic Pressure on Microbial Growth
Experiment 4: Effect of Oxygen on Microbial Growth
Antibiotic Sensitivity
Background
Procedure
Experiment 1: Performing a Disk Diffusion Test with E. coli
Experiment 2: Performing a Disk Diffusion Test with S. aureus
Experiment 3: Performing a Disk Diffusion Test with MRSA
Bacteria
Background
Procedure
Experiment 1: Performing Gram Staining of S. aureus and E. coli
-Part 1: Heat-Fixation of S. aureus
-Part 2: Gram Staining of S. aureus
-Part 3: Visualizing S. aureus with the Microscope
-Part 4: Repeating the Gram Staining Protocol with the E. coli
Experiment 2: Determining the Susceptibility of Gram-Positive and Gram-Negative Bacteria to Antibiotics
-Part 1: Performing a Disk Diffusion Test with Gram-Positive Bacteria
-Part 2: Repeat the Disk Diffusion Test with Gram-Negative Bacteria
Diversity
Protists
Background
Procedure
Topic 1: Investigating Excavata
-Part 1: Visualizing Euglena
-Part 2: Observing Euglena Motion
Topic 2: Investigating Rhizaria
-Part 1: Visualizing Foraminifera Shells
-Part 2: Observing Foram Motion
-Part 3: Investigating Radiolarians
Topic 3: Investigating Chromalveolata
-Part 1: Investigating Diatoms
-Part 2: Investigating Paramecium
-Part 3: Investigating Stentor
-Part 4: Investigating Dinoflagellates
Topic 4: Investigating Archaeplastida
-Part 1: Investigating Spirogyra
-Part 2: Investigating Volvox
-Part 3: Investigating Seaweed
Topic 5: Investigating Amoebas
Topic 6: Investigating Choanoflagellates
Fungi
Background
Procedure
Topic 1: Investigating Phylum Basidiomycota
-Part 1: Visualizing a Whole Agaricus Mushroom Specimen
-Part 2: Visualizing Agaricus Gills
-Part 3: Visualizing Agaricus Hyphae, Basidia, and Spores
Topic 2: Investigating Phylum Ascomycota
-Part 1: Visualizing Whole Ascomycota Mushroom Specimens
-Part 2: Visualizing Aspergillus
-Topic 3: Investigating Phylum Zygomycota
Chemistry
Acids, Bases, and pH Buffers
Background
Procedure
Experiment 1: Measuring pH by Using the pH Indicator Bromothymol Blue
Experiment 2: The Phosphate Buffer System
-Part 1: Measuring pH Changes Following the Addition of HCl
-Part 2: Measuring pH Changes Following the Addition of NaOH
Experiment 3: Measuring the Buffer Capacity of a Phosphate Buffer
-Part 1: Addition of Acid
-Part 2: Addition of Base
Macromolecules
Biological Molecules
Background
Procedure
Experiment 1: Testing for Reducing Sugars Using Benedict’s Solution
Experiment 2: Testing for Starch Using Lugol’s Iodine
Experiment 3: Testing for Lipids Using Sudan III Solution
Experiment 4: Testing for Proteins Using Biuret Solution
Experiment 5: Testing Various Foods for Reducing Sugars, Starch, Lipids, and Proteins
- Part 1: Testing Potato Juice
- Part 2: Testing Onion Juice
- Part 3: Testing Whole Milk
- Part 4: Testing Skim Milk
Enzymes
Background
Procedure
Experiment 1: Determining the Effect of Temperature on Catalase Activity
- Part 1: Determining the Effect of 10 °C on Catalase Activity
- Part 2: Determining the Effect of 21.5 °C on Catalase Activity
- Part 3: Determining the Effect of 40 °C on Catalase Activity
- Part 4: Determining the Effect of 60 °C on Catalase Activity
- Part 5: Determining the Effect of 80 °C on Catalase Activity
Experiment 2: Determining the Effect of Substrate Concentration on Catalase Activity
- Part 1: Testing the Catalase Activity of Test
-Tube 1
- Part 2: Testing the Catalase Activity of Test
-Tube 2
- Part 3: Testing the Catalase Activity of Test
-Tube 3
- Part 4: Testing the Catalase Activity of Test
-Tube 4
Experiment 3: Determining the Effect of pH on Catalase Activity
- Part 1: Testing Catalase Activity at pH 2
- Part 2: Testing Catalase Activity at pH 6
- Part 3: Testing Catalase Activity at pH 10
Quantitative Analysis of Enzyme Activity
Background
Procedure
Experiment 1: Creating a Calibration Curve for Starch–Iodine Measurements
- Part 1: Preparing a Set of Three Standards of Known Starch Concentration
- Part 2: Measuring Absorbance of the Three Standards
Experiment 2: Determining the Effect of pH on Amylase Enzyme Activity
- Part 1: Preparing the Reaction Solutions and Measuring Their pH
- Part 2: Measuring Absorbance of Test Tube 1 After an Amylase Hydrolysis Reaction
- Part 3: Measuring Absorbance of Test Tube 2 After an Amylase Hydrolysis Reaction
- Part 4: Measuring Absorbance of Test Tube 3 After an Amylase Hydrolysis Reaction
- Part 5: Measuring Absorbance of Test Tube 4 After an Amylase Hydrolysis Reaction
- Part 6: Measuring Absorbance of Test Tube 5 After an Amylase Hydrolysis Reaction
Experiment 3: Determining the Effect of Temperature on Amylase Enzyme Activity
- Part 1: Determining the Effect of 10 °C on Amylase Enzyme Activity
- Part 2: Determining the Effect of 37 °C on Amylase Enzyme Activity
- Part 3: Determining the Effect of 50 °C on Amylase Enzyme Activity
- Part 4: Determining the Effect of 80 °C onAmylase Enzyme Activity
Cells
Diffusion and Osmosis
Background
Procedure
Experiment 1: Qualitative Evidence for Diffusion
Experiment 2: Quantifying the Relationship Between Concentration Gradient and Osmosis
Experiment 3: Visualizing Osmosis in Living Cells
Expanded Diffusion and Osmosis
Background
Procedure
Experiment 1: Qualitative Evidence for Diffusion
Experiment 2: Observing the Dependence of the Rate of Diffusion on the Concentration Gradient
Experiment 3: Confirming Osmosis by Quantifying Weight Changes and Screening for Protein
- Part 1: Quantifying Weight Changes to Confirm Osmosis
- Part 2: Screening for the Presence of Protein with a Biuret Test to Confirm Osmosis
Experiment 4: Quantifying the Relationship Between Concentration Gradient and Osmosis
Metabolism
Cellular Respiration
Background
Procedure
Experiment 1: Fermentation of Different Sugars by Yeast Cells
- Part 1: Measuring Fermentation of Glucose
- Part 2: Measuring Fermentation of Fructose
- Part 3: Measuring Fermentation of Maltose
- Part 4: Measuring Fermentation of Maltotriose
Genetics
DNA
Background
Procedure
Experiment 1: Running a Gel Electrophoresis of DNA VNTR Fragments
Regulation of Gene Expression
Background
Procedure
Topic 1: Investigating Gene Expression
- Part 1: Transcribing Gene 1
- Part 2: Translating Gene 1
- Part 3: Regulating Gene 1
- Part 4: Transcribing Gene 2
- Part 5: Translating Gene 2
- Part 6: Regulating Gene 2
- Part 7: Transcribing Gene 3
- Part 8: Translating Gene 3
- Part 9: Regulating Gene 3
Topic 2: Investigating mRNA Production
Topic 3: Optimizing Protein Production Using Multiple Cells
Biotechnology
PCR
Background
Procedure
Experiment 1: Running a PCR
Experiment 2: Conducting Gel Electrophoresis
Nucleic Acid Assays
Background
Procedure
Experiment 1: Performing an RNA Extraction from Animal Cells
- Part 1: Resuspending the Pellet Containing RNA
- Part 2: Performing Cell Lysis
- Part 3: Homogenizing RNA
- Part 4: Purifying RNA
- Part 5: Summarizing the RNA Extraction Steps
Experiment 2: Cloning
Experiment 3: Next Generation Sequencing
- Part 1: Understanding Next Generation Sequencing
- Part 2: Preparing the Sample
- Part 3: Loading Components Into the MiSeq
- Part 4: Performing Cluster Generation
- Part 5: Sequencing by Synthesis
