Advanced Genetic Analysis: Finding Meaning in a Genome

by R. Scott Hawley (Stowers Institute for Medical Research); Michelle Walker (University of California, Davis)

• ISBN13: 9781444313086
• ISBN10: 1444313088
• Format: eBook
• Copyright: 2009-04-01
• Publisher: Wiley-Blackwell

Summary

Advanced Genetic Analysis brings a state-of-the-art, exciting new approach to genetic analysis. Focusing on the underlying principles of modern genetic analysis, this book provides the 'how' and 'why' of the essential analytical tools needed. The author's vibrant, accessible style provides an easy guide to difficult genetic concepts, from mutation and gene function to gene mapping and chromosome segregation. Throughout, a balanced range of model organisms and timely examples are used to illustrate the theoretical basics. Basic principles - Focuses students attention on the 'how' and 'why' of the essential analytical tools. Vibrant, accessible style provides an easy guide through difficult genetic concepts and techniques. Text boxes highlight key questions and timely examples. Boxes of key information in each chapter, chapter summaries and extensive references - prompt the student to synthesise and reinforce the chapter material. Special reference section addressing a range of model organisms to help provide a particularly relevant context for students' research interests.

Table of Contents

Preface

Introduction

Mutation: Types Of Mutations

Dominance And Recessivity

The Genetic Utility Of Dominant And Recessive Mutants

Summary

Gallery Of Model Organisms

Mutant Hunts: Why Look For New Mutants?

Mutagenesis And Mutational Mechanisms

What Phenotype Should You Screen (Or Select) For?

Actually Getting Started

Summary

The Complementation Test: The Essence Of The Complementation Test

Rules For Uing The Complementation Test

How Might The Complementation Test Lie To You?

Second-Site Non-Complementation (SSNC) (Non-Allelic Non-Complementation)

An Extension Of Second-Site Non-Complementation: Dominant Enhancers

Summary

Suppression: A Basic Definition Of Genetic Suppression

Intragenic Suppression (Pseudo-Reversion)

Extragenic Suppression

Transcriptional Suppression

Translational Suppression

Suppression By Post-Translational Modification

Extragenic Suppression As A Result Of Protein-Protein Interaction

Suppression Without Physical Interaction

Suppression Of Dominant Mutations

Designing Your Own Screen For Suppressor Mutations

Summary And A Warning

Determining When And Where Genes Function: Epistasis: Ordering Gene Function In Pathways

Mosaic Analysis: Where Does A Given Gene Act

Summary

Genetic Fine-Structure Analysis: Intragenic Mapping (Then)

Intragenic Mapping (Now)

Intragenic Complementation Meets Intragenic Recombination: The Basis Of Fine-Structure Analysis

An Example Of Fine-Structure Analysis For A Eukaryotic Gene Encoding A Multifunctional Protein

Fine-Structure Analysis Of Genes With Complex Regulatory Elements In Eukaryotes

Pairing-Dependent Intragenic Complementation

Summary

Meiotic Recombination: An Introduction To Meiosis

Crossingover And Chiasmata: Recombination Involves The Physical Interchange Of Genetic Material And Ensures Homolog Separation

The Classical Analysis Of Recombination

Measuring The Frequency Of Recombination

The Mechanism Of Recombination

Summary

Meiotic Chromosome Segregation: Types And Consequences Of Failed Segregation

The Origin Of Spontaneous Non-Disjunction

The Centromere

Segregational Mechanisms

Summary

Epilogue

References

Partial author index

Subject Index

Table of Contents provided by Publisher. All Rights Reserved.