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9780849321252

Advanced Methods in Cellular Immunology

by ;
  • ISBN13:

    9780849321252

  • ISBN10:

    0849321255

  • Edition: 1st
  • Format: Paperback
  • Copyright: 2000-05-26
  • Publisher: CRC Press

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Supplemental Materials

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Summary

Immunologists as well investigators in other disciplines may often use protocols involving the isolation, cultures and characterization of different types of leukocytes. Advanced Methods in Cellular Immunology is a collection of techniques in an easy-to-use format.Each chapter provides readers with related program information, a step-by-step description of the methodology, alternative techniques, pertinent references, and information about commercial sources for materials and regents. In addition to leukocytes, the authors guide readers through the processes of cell culture as well as inflammation and autoimmunity in a variety of animal models.Covering topics such as PCR and Apoptosis, this book will serve a guide to commonly used procedures in cellular immunology while utilizing both human and murine models.

Author Biography

Vaclav Vetvicka, Ph.D. is an assistant professor in the Department of Pathology, Division of Experimental Immunology and Immunopathology, School of Medicine, University of Louisville, Kentucky.

Table of Contents

Sources
1(28)
Isolation and characterization of neutrophils
29(12)
Isolation and characterization of eosinophils
41(8)
Enrichment of human basophils
49(2)
Macrophages
51(26)
B lymphocyte cloning
77(16)
Cell cultivation in vitro
93(8)
Analysis of cytokine or cytokine receptor mRNA
101(14)
Intracellular cytokine staining
115(4)
Cell cytotoxicity
119(16)
Mixed lymphocyte reaction
135(4)
Animal models of autoimmune diseases
139(16)
Buffers and media
155(8)
Commercial sources
163(8)
Index 171

Supplemental Materials

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