Amino Acid Analysis Protocols

by Cooper, Catherine; Packer, Nicolle; Williams, Keith

• ISBN13: 9780896036567
• ISBN10: 0896036561
• Format: Hardcover
• Copyright: 2000-09-01
• Publisher: Humana Pr Inc

Summary

A collection of classic and cutting-edge techniques of high utility in answering specific biological questions about amino acids. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Each method is described in step-by-step detail to ensure successful experimental results and emphasizes sample preparation, particularly the collection and storage of bodily fluids. Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Table of Contents

Preface	v
Contributors	ix
Amino Acid Analysis: An Overview	1	(8)
Margaret I. Tyler
Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory	9	(22)
Frank D. Macchi
Felicity J. Shen
Rodney G. Keck
Reed J. Harris
Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Anslysis	31	(8)
Li Zhang
Nancy Denslow
Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate	39	(10)
Steven A. Cohen
Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent)	49	(6)
Sunil Kochhar
Barbara Mouratou
Philipp Christen
The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection	55	(8)
C. David Forster
Charles A. Marsden
Anion Exchange Chromatography and Intergrated Amperometric Detection of Amino Acids	63	(24)
Petr Jandik
Christopher Pohl
Victor Barreto
Nebojsa Avdalovic
Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane	87	(14)
Noriko Shindo
Tsutomu Fujimura
Saiko Kazuno
Kimie Murayama
Capillary Gas Chromatographic Analysis of Protein and Nonprotein Amino Acids in Biological Samples	101	(22)
Hiroyuki Kataoka
Sayuri Matsumura
Shigeo Yamamoto
Masami Makita
Measurement of Blood Plasma Amino Acids in Ultrafiltrates by High-Performance Liquid Chromatography with Automatic Precolumn O-Phthaldialdehyde Derivatization	123	(18)
Hua Liu
Determination of Amino Acids in Foods by Reversed-Phase High-Performance Liquid Chromatography with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared to the Phenylthiocarbamyl Derivative and lon Exchange Chromatography	141	(28)
Kang-Lyung Woo
Amino Acid Measurement in Body Fluids Using PITC Derivatives	169	(8)
Roy A. Sherwood
Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials by Analysis of Phenylthiocarbamyl Derivatives	177	(6)
Margareta Stark
Jan Johansson
Analysis of O-Phosphoamino Acids in Biological Samples by Gas Chromatography with Flame Photometric Detection	183	(24)
Hiroyuki Kataoka
Norihisa Sakiyama
Yukizo Ueno
Kiyohiko Nakai
Masami Makita
Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection	207	(20)
Hiroyuki Kataoka
Kiyomi Takagi
Hirofumi Tanaka
Masami Makita
Capillary Electrophoretic Determination of 4-Hydroxyproline	227	(10)
Qingyi Chu
Michael Zeece
Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization	237	(8)
Isabella Fermo
Rita Paroni
Determination of Early Glycation Products by Mass Spectrometry and Quantification of Glycation Mediated Protein Crosslinks by the Incorporation of [14C]lysine into Proteins	245	(10)
Malladi Prabhakaram
Beryl J. Ortwerth
Jean B. Smith
Index	255