What is included with this book?
Cloning of the Antigen-binding Site from Hybridoma | |
Cloning of Variable Domains from Mouse Hybridoma by PCR | p. 3 |
Coning Hybridoma cDNA by RACE | p. 15 |
Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly | p. 21 |
Generation of Antibody Repertoires | |
Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens | p. 47 |
Human Antibody Gene Libraries | p. 65 |
Synthetic Antibody Libraries | p. 85 |
Immune Libraries from Nonhuman Primates (NHP) | p. 99 |
Generation of Rabbit Immune Libraries | p. 115 |
Selection of Antibody Fragments from Combinatorial Libraries | |
Immunotube Selections | p. 127 |
Phage Display and Selection in Microtitre Plates | p. 139 |
Phage Display and Selections on Biotinylated Antigens | p. 151 |
Phage Display and Subtractive Selection on Cells | p. 165 |
Selection of Phage Antibody Libraries for Binding and Internalization into Mammalian Cells | p. 183 |
Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA | p. 197 |
Yeast Display and Selections | p. 207 |
The Generation of Transgenic Mice Expressing Human Antibody Repertoires | p. 235 |
Selection of Antibody Fragments by Means of the Filter-Sandwich Colony Screening Assay | p. 255 |
Semi-automated Magnetic Bead-Based Antibody Selection from Phage Display Libraries | p. 267 |
Engineering and Production of Immunoglobulins | |
Aspects of Isotype Selection | p. 291 |
Generation of Heavy and Light Chains (Chimeric Antibodies) | p. 307 |
Humanising Antibodies by CDR Grafting | p. 319 |
Humanization by Resurfacing | p. 341 |
Human Antibodies by Guided Selection | p. 355 |
In Silico De-Immunization | p. 369 |
Affinity Maturation by Chain Shuffling and Site Directed Mutagenesis | p. 377 |
Affinity Maturation by Random Mutagenesis and Phage Display | p. 397 |
Engineering of the Fc Region for Improved PK (FcRn Interaction) | p. 411 |
Antibody-Dependent Enzyme Prodrug Therapy (ADEPT) | p. 431 |
Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System | p. 453 |
Expression of IgA Molecules in Mammalian Cells | p. 471 |
Antibody Characterization | |
Expression of Complete Antibodies in Transgenic Plants | p. 489 |
Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast | p. 503 |
Expression of IgG Antibodies in Mammalian Cells | p. 517 |
Protein A/G Chromatography | p. 531 |
Epitope Analysis Using Synthetic Peptide Repertoires Prepared by SPOT Synthesis Technology | p. 537 |
Epitope Mapping by Printed Peptide Libraries | p. 573 |
Antibody Epitope Mapping Using Yeast Display | p. 591 |
Size Exclusion Chromatography | p. 607 |
Structural Characterization of Antibodies by Mass Spectrometry | p. 613 |
Antibody Glycans Characterization | p. 635 |
Affinity Measurements by Competition ELISA | p. 657 |
Anti-Histidine Antibodies as Tools for Reversible Capturing of His-Tagged Fusion Proteins for Subsequent Binding Analysis | p. 667 |
Affinity Measurements Using Quartz Crystal Microbalance (QCM) | p. 683 |
Affinity Measurements with Radiolabeled Antibodies | p. 695 |
Neutralization Tests | p. 705 |
Functional Characterization of Antibodies Neutralizing Soluble Factors In Vitro and In Vivo | p. 723 |
Competitive ELISA | p. 739 |
Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA | p. 743 |
Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay | p. 749 |
Binding Studies with Flow Cytometry | p. 765 |
Index | p. 781 |
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