Antisense Technology, Part A, General Methods, Methods of Delivery, and RNA Studies

by Abelson; Simon; Phillips

• ISBN13: 9780121822149
• ISBN10: 0121822141
• Edition: 1st
• Format: Hardcover
• Copyright: 1999-10-25
• Publisher: Elsevier Science

Summary

Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Table of Contents

Contributors to Volume 313	ix
Preface	xiii
Volumes in Series	xv
Section I. General Methods
Progress in Antisense Technology: The End of the Beginning	3	(43)
Stanley T. Crooke
Basic Principles of Using Antisense Oligonucleotides in Vivo	46	(10)
M. Ian Phillips
Y. Clare Zhang
Polyethyleneimine-Mediated Transfection to Improve Antisense Activity of 3'-Capped Phosphodiester Oligonucleotides	56	(18)
Sonia Dheur
E. Tula Saison-Behmoaras
Design of Antisense and Triplex-Forming Oligonucleotides	74	(21)
Jean-Christophe Francois
Jerome Lacoste
Laurent Lacroix
Jean-Louis Mergny
Chimeric Oligodeoxynucleotide Analogs: Chemical Synthesis, Purification, and Molecular and Cellular Biology Protocols	95	(40)
Richard V. Giles
David G. Spiller
David M. Tidd
Evaluation of Antisense Mechanisms of Action	135	(8)
Chandramallika Ghosh
David Stein
Dwight Weller
Patrick Iversen
Physiology and Molecular Biology Brought to Single-Cell Level	143	(13)
Pierre-Marie Lledo
David Desmaisons
Alan Carleton
Jean-Didier Vincent
Antisense Properties of Peptide Nucleic Acid	156	(8)
Peter E. Nielsen
Synthesis of Oligonucleotide Conjugates in Anhydrous Dimethyl Sulfoxide	164	(10)
David Milesi
Igor Kutyavin
Eugene A. Lukhtanov
Vladimir V. Gorn
Michael W. Reed
Gene Switching: Analyzing Broad Range of Mutations Using Steric Block Antisense Oligonucletides	174	(15)
Paul A. Morcos
Vesicular Stomatitis Virus as Model System for Studies of Antisense Oligonucleotide Translation Arrest	189	(14)
Ian Robbins
Bernard Lebleu
Purification of Antisense Oligonucleotides	203	(24)
Ranjit R. Deshmukh
Douglas L. Cole
Yogesh S. Sanghvi
Boranophosphate Backbone: A Mimic of Phosphodiesters, Phosphorothioates, and Methyl Phosphonates	227	(30)
Barbara Ramsay Shaw
Dmitri Sergueev
Kaizhang He
Ken Porter
Jack Summers
Zinaida Sergueeva
Vladimir Ratt
Intracellular Distribution of Digoxigenin-Labeled Phosphorothiate Oligonucleotides	257	(11)
Gemma Tarrason
David Bellido
Ramon Eritja
Senen Vilaro
Jaume Piulats
Use of Minimally Modified Antisense Oligonucleotides for Specific Inhibition of Gene Expression	268	(19)
Eugen Uhlmann
Anusch Peyman
Antonina Ryte
Annette Schmidt
Eckhart Buddecke
Section II. Methods of Delivery
Determination of Cellular Internalization of Fluorescent Oligonucleotides	287	(10)
Lyuba Benimetskaya
John Tonkinson
C.A. Stein
Intrabody Tissue-Specific Delivery of Antisense Conjugates in Animals: Ligand-Linker-Antisense Oligomer Conjugates	297	(25)
Robert J. Duff
Scott F. Deamond
Clinton Roby
Yuanzhong Zhou
Paul O. P. Ts'o
Lipid-Based Formulations of Antisense Oligonucleotides for Systemic Delivery Applications	322	(20)
Sean C. Semple
Sandra K. Klimuk
Troy O. Harasym
Michael J. Hope
In Vitro Transport and Delivery of Antisense Oligonucleotides	342	(17)
Jeff Hughes
Anna Astriab
Hoon Yoo
Suresh Alahari
Earvin Liang
Dmitri Sergueev
Barbara Ramsay Shaw
R. L. Juliano
In Vitro and in Vivo Delivery of Antisense Oligodeoxynucleotides Using Lipofection: Application of Antisense Technique to Growth Suppression of Experimental Glioma	359	(13)
Akira Matsuno
Tadashi Nagashima
Haruko Katayama
Akira Tamura
Preparation and Application of Liposome-Incorporated Oligodeoxynucleotides	372	(16)
Ana M. Tari
Cell-Specific Optimization of Phosphorothioate Antisense Oligodeoxynucleotide Delivery by Cationic Lipids	388	(13)
Shirley A. Williams
Jeffrey S. Buzby
Section III. RNA Studies
Intracellular Expression and Function of Antisense Catalytic RNAs	401	(19)
Daniela Castanotto
Michaela Scherr
John J. Rossi
Selective Degradation of Targeted m RNAs Using Partially Modified Oligonucleotides	420	(16)
John M. Dagle
Daniel L. Weeks
Reversible Depletion of Specific RNAs by Antisense Oligodeoxynucleotide-Targeted Degradation in Frog Oocytes	436	(6)
George L. Eliceiri
Inactivation of Gene Expression Using Ribonuclease P and External Guide Sequences	442	(14)
Cecilia Guerrier-Takada
Sidney Altman
Disruption of mRNA-RNP Formation and Sorting to Dendritic Synapses by Antisense Oligonucleotides	456	(11)
Emma R. Jakoi
W. L. Severt
Antisense RNA and DNA in Escherichia coli	467	(18)
Oleg Mirochnitchenko
Masayori Inouye
Utilization of Properties of Natural Catalytic RNA to Design and Synthesize Functional Ribozymes	485	(21)
Leonidas A. Phylactou
Charlotte Darrah
Louise Everatt
Despina Maniotis
Michael W. Kilpatrick
Antisense Oligonucleotides and RNAs as Modulators of pre-mRNA Splicing	506	(16)
Halina Sierakowska
Linda Gorman
Shin-Hong Kang
Ryszard Kole
Selective RNA Cleavage by Isolated RNase L Activated with 2-5A Antisense Chimeric Oligonucleotides	522	(13)
Robert H. Silverman
Beihua Dong
Ratan K. Maitra
Mark R. Player
Paul F. Torrence
Author Index	535	(32)
Subject Index	567