Antisense Technology A Practical Approach

by Lichtenstein, Conrad; Nellen, Wolfgang

• ISBN13: 9780199635832
• ISBN10: 0199635838
• Format: Paperback
• Copyright: 1998-03-05
• Publisher: IRL Press

Summary

This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition, it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic acid structures; measurement and evaluation of antisense effects; selection, preparation, and the use of antisense oligonucleotides; in vitro RNA transcription; construction strategies, testing and optimization of catalytic antisense RNAs based on hammerhead ribozymes; synthesis and evaluation of 2-5 A- antisense chimeras for targeted degradation of RNA. The numerous hints and tips for success and advice on trouble shooting, make this authoritative text invaluable to all those researchers who work on gene expression, RNA transcription, or protein function.

Table of Contents

Nucleic acid structures

Evaluation of antisense effects

Selecting, preparing, and handling antisense oligodeoxyribonucleotides

In vitro RNAs

Catalytic antisense RNA based on hammerhead ribozymes

2-5 A-Antisense chimaeras for targeted degradation of RNA

In vitro applications of antisense RNA and DNA

Antisense applications in Dictyostelium: a lower eukaryotic model system

Applications of antisense technology in plants

Antisense molecules and ribozymes: medical applications

Non-antisense effects of oligodeoxynucleotides

Antisense rescue

Shotgun antisense mutants

Detection of sense-antisense duplexes by structure-specific anti-RNA antibodies

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