Application of Solution Protein Chemistry to Biotechnology

by Lundblad; Roger L.

• ISBN13: 9781420073416
• ISBN10: 1420073419
• Edition: 1st
• Format: Hardcover
• Copyright: 2009-05-12
• Publisher: CRC Press

Summary

Relying upon his broad view of biotechnology, pioneering scientist, Roger Lundblad draws from both traditional protein science and proteomics to include a great deal of material not found in electronic format. He includes chapters on the use of classical protein chemistry in food, the protein and carbohydrate chemistry of adhesives, glues and sealants, protein chemistry and hydrogels, and the use of chemical modification to prepare protein therapeutics. While demonstrating current use and the development of biotech applications, Lundblad makes a point to separate what is truly new from that which has merely been renamed. Ultimately, he provides much time saving research on which others can build.One of the world's most respected protein researcher, Dr. Lundblad has authrored and edited several books for CRC Press including theBiochemistry and Molecular Biology Compendium.

Author Biography

Dr. Lundblad also goes far beyond the biopharmaceutical applications that are often equated with protein science today to demonstrate the field's unique versatility. From the making of bread and the invention of adhesives to the production of pharmaceuticals and the development of recombinant DNA products-in each of these products, the role of the protein chemist remains prominent. The important point is that classical protein chemistry is a critical part of the practice of biotechnology in the marketplace.

Table of Contents

Preface	p. ix
The Author	p. xi
Introduction to the Solution Chemistry of Proteins	p. 1
Amino Groups	p. 21
Tyrosine	p. 39
Cystine	p. 62
Methionine	p. 65
Tryptophan	p. 68
Arginine	p. 72
Histidine	p. 75
Carboxyl Groups	p. 78
Chemical Cleavage of Peptide Chains	p. 84
References	p. 87
Application of Solution Protein Chemistry to the Study of Biopharmaceutical Conformation	p. 131
References	p. 143
Chemistry of the Attachment of Proteins and Proteins and Peptides to Solid Surfaces	p. 163
DNA and Protein Microarray	p. 163
Solid-Phase Matrices (Including Beads) for Attachment of Protein Probes	p. 165
Protein Interaction with Steel 100-108 and Titanium 109-115	p. 165
Chemistry for Attachment of Proteins and Peptides to Solid-Phase Matrices	p. 168
References	p. 178
Protein Conjugates	p. 195
Introduction	p. 195
Protein Conjugates	p. 198
Albumin Bioconjugates	p. 204
Antibody-Protein Conjugates	p. 209
Direct Labeling of Antibodies with Radioisotopes	p. 213
Antibody-Drug	p. 213
Antibody-Radiolabel	p. 217
Protein-Carbohydrate Conjugates	p. 217
Polyethylene Glycol	p. 220
References	p. 230
Protein Hydrogels	p. 251
References	p. 254
Adhesives, Glues, and Sealants	p. 281
Tissue Soldering	p. 283
Proteins as Tissue Solder Material	p. 285
Collagen	p. 285
Albumin	p. 286
Fibrinogen	p. 288
Fibrin Sealant	p. 289
Gelatin-Resorcinol-Formaldehyde and Gelatin-Resorcinol-Formaldehyde-Glutaraldehyde	p. 294
BioGlue$$	p. 297
Mussel Adhesive Protein	p. 297
End Notes	p. 298
References	p. 299
Protein Drug Delivery	p. 327
References	p. 332
Application of Solution Protein Chemistry to Protemics	p. 339
References	p. 368
Use of Chemical Modification to Produce Biopharmaceutical Products	p. 379
Chemical Modification of Oligosaccharides/Polysaccharides to Produce Therapeutic Products	p. 380
Chemical Modification of Nucleic Acids	p. 381
Chemical Modification and the Manufacture of Therapeutic Proteins	p. 385
Chemical Glycosylation	p. 385
Allergoids	p. 389
Cross-Linkage	p. 391
Formaldehyde	p. 394
Active-Site Blocked Enzymes as Competitive Inhibitors	p. 395
Miscellaneous Chemical Modification of Proteins Having Therapeutic Value	p. 397
References	p. 399
Food and Agricultural Chemistry	p. 411
References	p. 420
Index	p. 433
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