Applications of Chimeric Genes and Hybrid Proteins, Part B: Cell Biology and Physiology

by Simon; Thorner; Emr; Abelson

• ISBN13: 9780121822286
• ISBN10: 0121822281
• eBook ISBN(s): 9780080496825, 9780121822286
• Edition: 1st
• Format: Hardcover
• Copyright: 2000-10-09
• Publisher: Elsevier Science
• More Book Details
• Purchase Benefits

Summary

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Table of Contents

Contributors to Volume 327	xi
Preface	xvii
Volumes in Series	xix
Section I. Epitope Tags for Immunodetection
Epitope Tagging: General Method for Tracking Recombinant Proteins	3	(16)
Christian E. Fritze
Thomas R. Anderson
Section II. Markers for Cytology, Analysis of Protein Trafficking, and Lineage Tracing
Alkaline Phosphatase Fusions of Ligands or Receptors as in Situ Probes for Staining of Cells, Tissues, and Embroys	19	(16)
John G. Flanagan
Hwai-Jong Cheng
David A. Feldheim
Mitsuharu Hattori
Qiang Lu
Pierre Vanderhaeghen
Chimeric Molecules Employing Horseradish Peroxidase as Reporter Enzyme for Protein Localization in the Electron Microscope	35	(10)
Colin Hopkins
Adele Gibson
Jane Stinchcombe
Clare Futter
Biochemical Analyses of Trafficking with Horseradish Peroxidase-Tagged Chimeras	45	(16)
Anastasiya D. Blagoveshchenskaya
Daniel F. Cutler
Visualizing Protein Dynamics in Yeast with Green Fluorescent Protein	61	(8)
Christopher G. Burd
Kinetic Analysis of Intracellular Trafficking in Single Living Cells with Vesicular Stomatitis Virus Protein G-Green Fluorescent Protein Hybrids	69	(20)
Koret Hirschberg
Robert D. Phair
Jennifer Lippincott-Schwartz
Dual Color Detection of Cyan and Yellow Derivatives of Green Fluorescent Protein Using Conventional Fluorescence Microscopy and 35-mm Photography	89	(6)
Gisele Green
Steven R. Kain
Brigitte Angres
Invertase Fusion Proteins for Analysis of Protein Trafficking in Yeast	95	(12)
Tamara Darsow
Greg Odorizzi
Scott D. Emr
Introduction of Kex2 Cleavage Sites in Fusion Proteins for Monitoring Localization and Transport in Yeast Secretory Pathway	107	(11)
B. Diane Hopkins
Ken Sato
Akihiko Nakuno
Todd R. Graham
Lineage Analysis with Retroviral Vectors	118	(27)
Constance L. Cepko
Elizabeth Ryder
Christopher Austin
Jeffrey Golden
Shawn Fields-Berry
John Lin
Use of Pseudotyped Retroviruses in Zebrafish as Genetic Tags	145	(20)
Shawn Burgess
Nancy Hopkins
Section III. Tools for Analysis of Membrane Proteins
A Gene Fusion Method for Assaying Interactions of Protein Transmembrane Segments in Vivo	165	(10)
Jennifer A. Leeds
Jon Beckwith
Using SUC2-HIS4C Reporter Domain to Study Topology of Membrane Proteins in Saccharomyces cerevisiae	175	(15)
Christian Sengstag
Detecting Interactions between Membrane Proteins in Vivo Using Chimeras	190	(8)
Igor Stagliar
Stephan te Heesen
Alkaline Phosphatase Fusion Proteins for Molecular Characterization and Cloning of Receptors and Their Ligands	198	(12)
John G. Flanagan
Hwai-Jong Cheng
Surface Chimeric Receptors as Tools in Study of Lymphocyte Activation	210	(18)
Bryan A. Irving
Arthur Weiss
Use of Chimeric Receptor Molecules to Dissect Signal Transduction Mechanisms	228	(11)
Warren J. Leonard
Fusion Protein Toxins Based on Diphtheria Toxin: Selective Targeting of Growth Factor Receptors of Eukaryotic Cells	239	(10)
Johanna C. VanderSpek
John R. Murphy
Green Fluroescent Protein-Based Sensors for Detecting Signal Transduction and Monitoring Ion Channel Function	249	(11)
Micah S. Siegel
Ehud Y. Isacoff
Metabolic Labeling of Glycoproteins with Chemical Tags through Unnatural Sialic Acid Biosynthesis	260	(19)
Christina L. Jacobs
Kevin J. Yarema
Lara K. Mahal
David A. Nauman
Neil W. Charters
Carolyn R. Bertozzi
Section IV. Signals for Addressing Proteins to Specific Subcellular Compartments
Using Sorting Signals to Retain Proteins in Endoplasmic Reticulum	279	(4)
Hugh R. B. Pelham
Directing Proteins to Nucleus by Fusion to Nuclear Localization Signal Tags	283	(14)
Heike Krebber
Pamela A. Silver
Identification, Analysis, and Use of Nuclear Export Signals in Saccharomyces cerevisiae	297	(8)
Karsten Weis
Directing Proteins to Mitochondria by Fusion to Mitochondrial Targeting Signals	305	(12)
Kostas Tokatlidis
Targeting Proteins to Plasma Membrane and Membrane Microdomains by N-Terminal Myristoylation and Palmitoylation	317	(14)
Wouter van't Hof
Marilyn D. Resh
Analysis of Function and Regulation of Proteins That Mediate Signal Transduction by Use of Lipid-Modified Plasma Membrane-Targeting Sequences	331	(20)
Gary W. Reuther
Janice E. Buss
Lawrence A. Quilliam
Geoffrey J. Clark
Channing J. Der
Glycerolphosphoinositide Anchors for Membrane-Tethering Proteins	351	(18)
John D. Fayen
Mark L. Tykocinski
M. Edward Medof
Fusions to Members of Fibroblast Growth Factor Gene Family to Study Nuclear Translocation and Nonclassic Exocytosis	369	(16)
I. Prudovsky
M. Landriscina
R. Soldi
S. Bellum
D. Small
V. Andreeva
Thomas Maciag
Section V. Application of Chimeras in Monitoring and Manipulating Cell Physiology
Posttranslational Regulation of Proteins by Fusions to Steroid-Binding Domains	385	(16)
Didier Picard
Tet Repressor-Based System for Regulated Gene Expression in Eukaryotic Cells: Principles and Advances	401	(20)
Udo Baron
Hermann Bujard
Coumermycin-Induced Dimerization of GyrB-Containing Fusion Proteins	421	(8)
Michael A. Farrar
Steven H. Olson
Roger M. Perlmutter
Use of Glutathione S-Transferase and Break Point Cluster Region Protein as Artificial Dimerization Domains to Activate Tyrosine Kinases	429	(11)
Yoshiro Maru
Recombinant Aequorin as Tool for Monitoring Calcium Concentration in Subcellular Compartments	440	(16)
Valerie Robert
Paolo Pinton
Valeria Tosello
Rosario Rizzuto
Tullio Pozzan
Recombinant Aequorin as Reporter of Changes in Intracellular Calcium in Mammalian Cells	456	(16)
Jenny Stables
Larry C. Matheakis
Ray Chang
Stephen Rees
Monitoring Protein Conformations and Interactions by Fluorescence Resonance Energy Transfer between Mutants of Green Fluorescent Protein	472	(28)
Atsushi Miyawaki
Roger Y. Tsien
Studies of Signal Transduction Events Using Chimeras to Green Fluorescent Protein	500	(13)
Tobias Meyer
Elena Oancea
Use of Fusions to Green Fluorescent Protein in the Detection of Apoptosis	513	(9)
Eve Shinbrot
Collin Spencer
Valerie Natale
Steven R. Kain
Synapto-pHluorins: Chimeras between pH-Sensitive Mutants of Green Fluorescent Protein and Synaptic Vesicle Membrane Proteins as Reporters of Neurotransmitter Release	522	(24)
Rafael Yuste
Rebecca B. Miller
Knut Holthoff
Shifang Zhang
Gero Miesenbock
Studying Organelle Physiology with Fusion Protein-Targeted Avidin and Fluorescent Biotin Conjugates	546	(19)
Minnie M. Wu
Juan Llopis
Stephen R. Adams
J. Michael McCaffery
Ken Teter
Markku S. Kulomaa
Terry E. Machen
Hsiao-Ping H. Moore
Roger Y. Tsien
Fluorescent Labeling of Recombinant Proteins in Living Cells with FIAsH	565	(13)
B. Albert Griffin
Stephen R. Adams
Jay Jones
Roger Y. Tsien
Ubiquitin Fusion Technique and Its Descendants	578	(16)
Alexander Vashavsky
Use of Phosphorylation Site Tags in Proteins	594	(21)
Sidney Pestka
Lei Lin
Wei Wu
Lara Izotova
Author Index	615	(42)
Subject Index	657