Applications of Chimeric Genes and Hybrid Proteins, Part C: Protein-Protein Interactions and Genomics

by Simon; Thorner; Emr; Abelson

• ISBN13: 9780121822293
• ISBN10: 012182229X
• Edition: 1st
• Format: Hardcover
• Copyright: 2000-10-24
• Publisher: Elsevier Science

Summary

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Table of Contents

Contributors to Volume 328	ix
Preface	xiii
Volumes in Series	xv
Section I. Applications of Two-Hybrid Methods and Related Techniques for Analysis of Protein-Protein Interactions
High-Throughput Screening for Protein-Protein Interactions Using Two-Hybrid Assay	3	(11)
Gerard Cagney
Peter Uetz
Stanley Fields
LexA-Based Two-Hybrid Systems	14	(12)
Sarah J. Fashena
Ilya G. Serebriiskii
Erica A. Golemis
Interaction Mating Methods in Two-Hybrid Systems	26	(21)
Mikhail G. Kolonin
Jinhui Zhong
Russell L. Finley, Jr.
Analysis and Identification of Protein-Protein Interactions Using Protein Recruitment Systems	47	(12)
Ami Aronheim
Michael Karin
A Bacterial Two-Hybrid System that Exploits a cAMP Signaling Cascade in Escherichia coli	59	(15)
Gouzel Karimova
Agnes Ullmann
Daniel Ladant
A Green Fluorescent Protein-Based Reverse Two-Hybrid System: Application to the Characterization of Large Numbers of Potential Protein-Protein Interactions	74	(15)
Hideki Endoh
Albertha J. M. Walhout
Marc Vidal
Yeast Three-Hybrid System for Detecting Ligand-Receptor Interactions	89	(14)
Eric C. Griffith
Edward J. Licitra
Jun O. Liu
Use of a Yeast Three-Hybrid System to Clone Bridging Proteins	103	(8)
Jie Zhang
The Yeast Tribrid System: cDNA Expression Cloning of Protein Interactions Dependent on Posttranslational Modifications	111	(17)
Jarema P. Kochan
Christoph Volpers
Mark A. Osborne
The Yeast Split-Hybrid System	128	(13)
Anthony J. DeMaggio
Phyllis Goldman
Hsiu-Ming Shih
Richard H. Goodman
Merl F. Hoekstra
Section II. Other Approaches Using Chimeras for Identification and Analysis of Interactions of Proteins and Nucleic Acids
Mapping Protein-Protein Interaction Domains Using Ordered Fragment Ladder Far-Western Analysis of Hexahistidine-Tagged Fusion Proteins	141	(16)
Richard R. Burgess
Terrance M. Arthur
Bradley C. Pietz
Mapping Specificity Determinants for Protein-Protein Association Using Protein Fusions and Random Peptide Libraries	157	(14)
Michael B. Yaffe
Lewis C. Cantley
Selection of Genetic Agents from Random Peptide Aptamer Expression Libraries	171	(37)
C. Ronald Geyer
Roger Brent
Detection of Protein-Protein Interactions by Protein Fragment Complementation Strategies	208	(23)
Stephen W. Michnick
Ingrid Remy
Francois-X. Campbell-Valois
Alexis Vallee-Belisle
Joelle N. Pelletier
Monitoring Protein-Protein Interactions in Live Mammalian Cells by β-Galactosidase Complementation	231	(20)
Fabio M. V. Rossi
Bruce T. Blakely
Carol A. Charlton
Helen M. Blau
Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions	251	(10)
Sang-Hyun Park
Ronald T. Raines
Protein Fusions to Coiled-Coil Domains	261	(21)
Kristian M. Muller
Katja M. Arndt
Tom Alber
Molecular Applications of Fusions to Leucine Zippers	282	(15)
Jennifer D. Rieker
James C. Hu
Using the Yeast Three-Hybrid System to Detect and Analyze RNA-Protein Interactions	297	(25)
Brian Kraemer
Beilin Zhang
Dhruba Sengupta
Stanley Fields
Marvin Wickens
Principles and Applications of a Tat-Based Assay for Analyzing Specific RNA-Protein Interactions in Mammalian Cells	322	(11)
Bryan R. Cullen
Section III. Phage Display and Its Applications
Phage Display for Selection of Novel Binding Peptides	333	(31)
Sachdev S. Sidhu
Henry B. Lowman
Brian C. Cunningham
James A. Wells
Selectively Infective Phage Technology	364	(25)
Katja M. Arndt
Sabine Jung
Claus Krebber
Andreas Pluckthun
Use of Phage Display and Transition-State Analogs to Select Enzyme Variants with Altered Catalytic Properties: Glutathione Transfease as an Example	389	(15)
Mikael Widersten
Lars O. Hansson
Lisa Tronstad
Bengt Mannervik
Selecting and Evolving Functional Proteins in Vitro by Ribosome Display	404	(26)
Jozef Hanes
Lutz Jermutus
Andreas Pluckthun
Yeast Surface Display for Directed Evolution of Protein Expression, Affinity, and Stability	430	(17)
Eric T. Boder
K. Dane Wittrup
Section IV. Construction of Hybrid Molecules by DNA Shuffling and Other Methods
Methods for in Vitro DNA Recombination and Random Chimeragenesis	447	(9)
Alexander A. Volkov
Frances H. Arnold
Random Chimeragenesis by Heteroduplex Recommbination	456	(7)
Alexander A. Volkov
Zhixin Shao
Frances H. Arnold
Use of Chimeras Generated by DNA Shuffling: Probing Structure-Function Relationships among Glutathione Transferases	463	(15)
Lars O. Hansson
Bengt Mannervik
Protein Engineering by Expressed Protein Ligation	478	(21)
Ulrich K. Blaschke
Jonathan Silberstein
Tom W. Muir
Section V. Applications of Fusions in Functional Genomics
A Systematic and General Proteolytic Method for Defining Structural and Functional Domains of Proteins	499	(16)
Jannette Carey
High-Throughput Expression of Fusion Proteins	515	(15)
Marc S. Nasoff
Mark Bergseid
James P. Hoeffler
John A. Heyman
Rapid Construction of Recombinant DNA by the Univector Plasmid-Fusion System	530	(20)
Qinghua Liu
Mamie Z. Li
Dou Liu
Stephen J. Elledge
High-Throughput Methods for the Large-Scale Analysis of Gene Function by Transposon Tagging	550	(25)
Anuj Kumar
Shelley Ann Des Etages
Paulo S. R. Coelho
G. Shirleen Roeder
Michael Snyder
Gateway Recombinational Cloning: Application to the Cloning of Large Numbers of Open Reading Frames or ORFeomes	575	(17)
Albertha J. M. Walhout
Gary F. Temple
Michael A. Brasch
James L. Hartley
Monique A. Lorson
Sander van den Heuvel
Marc Vidal
Gene Trapping Methods for the Identification and Functional Analysis of Cell Surface Proteins in Mice	592	(25)
William C. Skarnes
Author Index	617	(32)
Subject Index	649