Aqueous Two-Phase Systems

by Abelson; Simon; Walter; Johansson

• ISBN13: 9780121821296
• ISBN10: 0121821293
• Format: Hardcover
• Copyright: 1994-04-18
• Publisher: Elsevier Science

Summary

This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key Features * Use of affinity partitioning for enzyme purification * Separation and study of cells * Isolation of plasma membranes * Large scale biotechnical use

Table of Contents

General Methodology and Apparatus

Phase Diagrams

Preparation and Analysis of Two-Phase Systems

Partitioning Procedures and Techniques: Small Molecules and Macromolecules

Partitioning Procedures and Techniques: Cells, Organelles, and Membranes

Uses of Poly(ethylene Glycol) with Charged or Hydrophobic Groups

Systems Containing Organic Solvents

Chiral Resolution

Thin-Layer Countercurrent Distribution and Centrifugal Countercurrent Distribution Apparatus

Columns Using Aqueous Two-Phase Systems

Magnetically Enhanced Phase Separation

Partitioning and Affinity Partitioning of Macromolecules: Proteins

Affinity Extraction with Dye Ligands

Purification of Lactate Dehydrogenase from Pig Muscle by Affinity Partitioning

Phosphofructokinase from Baker's Yeast

Partitioning of Blood Proteins Using Immobilized Dyes

Metal Affinity Partitioning

Starch Synthase from Potato Tubers

Isolation of Integral Membrane Proteins by Phase Partitioning with Triton X-114. Nucleic Acids

Separation of Proteins and Nucleic Acids

Purification of Nucleic Acid-Protein Complexes

Studies on Protein Interactions

Molecular Structure, Charge, Hydrophobicity, and Conformational Changes

Cross-Partitioning: Determination of Isoelectric Point by Partitioning

Charge Determination by Partitioning

Protein(Protein and Protein(Ligand Interactions

Estimation of Surface Hydrophobicity of Proteins by Partitioning

Detection of Conformational Changes in Proteins by Probing with Poly(ethylene Glycol)-Bound Ligands

Analysis of Protein Structural Changes in Steroid Receptors by Partitioning

Use of Partitioning in Predicting Mild Hydrophobic Interaction Chromatography Behavior

Partitioning and Affinity Partitioning of Particulates

Cells Separation and Fractionation of Cell Populations and Obtaining Information on Their Surface Properties

Analytical Applications of Partitioning: Detection of Differences or Changes in Surface Properties of Mammalian Cell Populations

Cell-Cell Affinity

Testing for Charge and Hydrophobicity Correlates in Cell-Cell Adhesion

Surface Changes during Growth and Development of Slime Molds

Separation of Pollen from Nicotiana tabacum

Classification of Penicillium Fungi by Cross-Partition Analysis

Affinity Partitioning

Charge-Directed Affinity Partitioning of Cells

Factors in the A

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