Artificial DNA: Methods and Applications

by Khudyakov; Yury E.

• ISBN13: 9780849314261
• ISBN10: 0849314267
• Edition: 1st
• Format: Hardcover
• Copyright: 2002-09-25
• Publisher: CRC Press

Summary

Combining elements of biochemistry, molecular biology, and immunology, artificial DNA can be employed in a number of scientific disciplines. Some of the varied applications include site-specific mutagenesis, hybridization, amplification, protein engineering, anti-sense technology, DNA vaccines, protein vaccines, recombinant antibodies, screening for genetic and pathogenic diseases, development of materials with new biochemical and structural properties, and many more.Artificial DNA: Methods and Applications introduces the concept of artificial DNA that has been rationally designed and explains how it may be exploited in order to develop products that will achieve your intended purpose. The first part of the book covers methods of oligonucleotide synthesis and direct applications of synthetic DNA. The second part describes methods of gene assembly from synthetic oligonucleotides and applications of synthetic genes. The authors also discuss the different trends and future developments within each application area .With state-of-the art research, the contributing authors describe how to engineer proteins using rational and semi-rational design to exhibit the desired traits and detail the various amplification reactions and hybridization techniques for modeling evolution and for use in basic research. The only text devoted to this subject, Artificial DNA offers a comprehensive review that allows you to understand the strategy, design, and applications of synthetic oligonucleotides.

Table of Contents

Chemical Synthesis of Oligonucleotides: From Dream to Automation, Richard T. Pon

Artificial Hybridization Probes, Salvatore A.E. Marras

Peptide Nucleic Acid (PNA): A DNA Mimic with a Pseudopeptide Backbone, Frederik Beck and Peter E. Nielsen

Synthetic DNA Used in Amplification Reactions, Lisa S. Kelly

Artificial Nucleic Acids and Genomic Profiling, Lilia M. Ganova-Raeva

Site-Directed Mutagenesis, John I. Glass and Beverly A. Heinz

Mimotopes, Renu Tuteja

Artificial Genes for Chimeric Virus-like Particles, Paul Pumpens and Elmar Grens

DNA Vaccines, Sang Won Han, Jane Zveiter de Moraes, CTlio Lopes Silva, Roger Chammas,and Muaurfcio Martins Rodrfgues

Towards Designer Diagnostic Antigens, Joy Chih-Wei Chang

Recombinant Antibodies, Ramon Montana and Flor H. Pujol