Basic Laboratory Methods for Biotechnology

by Seidman, Lisa A.; Moore, Cynthia J.

• ISBN13: 9780321570147
• ISBN10: 0321570146
• Edition: 2nd
• Format: Spiral Bound
• Copyright: 2008-10-29
• Publisher: PEARSON

Summary

Presented from the perspective of the biotech industry, this laboratory handbook/textbook reference gives a systematic, understandable, and practical introduction to fundamental laboratory methods and provides a foundation upon which students can build a career in the lab. The authors balance background and theory with practical information, drawing material from many sources: analytical chemistry texts, molecular biology manuals, industry standards, government regulations, manufacturer and supplier information, and the useful laboratory "lore" that is part of the industryrs"s oral tradition. The Modern Biotechnology Industry: A Broad Overview, The Business of Biotechnology: The Transformation of Knowledge into Products, Pharmaceutical/Biopharmaceutical Products, Introduction to Product Quality Systems, Biotechnology and the Regulation of Food and Medical Products, Documentation, the Foundation of Quality, Quality Systems in the Production Facility, Quality Systems in the Laboratory, Introduction to a Safe Workplace, Working Safely in the Laboratory: General Considerations and Physical Hazards, Working Safely with Chemicals, Working Safely with Biological Materials, Basic Math Techniques, Proportional Relationships, Relationships and Graphing, Descriptions of Data (Descriptive Statistics),Introduction to Quality Laboratory Measurements, Tests and Assays, Introduction to Instrumental Methods and Electricity,The Measurement of Weight, The Measurement of Volume, The Measurement of Temperature, The Measurement of pH, Selected Ions and Conductivity, Measurements Involving Light A. Basic Principles and Instrumentation, Introduction to Quality Laboratory Tests and Assays, Measurements Involving Light B. Applications and Methods, Preparation of Laboratory Solutions A: Concentration Expressions and Calculations, Preparation of Laboratory Solutions B. Basic Procedures and Practical Information, Solutions: Associated Procedures and Information, Laboratory Solutions to Support the Activity of Biological Macromolecules, Culture Media for Intact Cells, Introduction to Filtration, Introduction to Centrifugation, Introduction to Bioseparations, Computers: An Overview, Data Handling with Computers, Applications of the Internet to Biotechnology. Itended for those interested in learning the basics of laboratory methods for biotechnology

Author Biography

Lisa Seidman received her Ph.D. from the University of Wisconsin and has taught for more than 20 years in the Biotechnology Laboratory Technician Program at Madison Area Technical College in Madison, Wisconsin. Cynthia Moore received her Ph.D. in Microbiology from Temple University School of Medicine. She is currently an Associate Professor of Biological Sciences at Illinois State University, where she also serves as Director of Biology Teacher Education.

Table of Contents

Introduction to the Biotechnology Work Place

The Modern Biotechnology Industry: A Broad Overview

The Business of Biotechnology: The Transformation of Knowledge into Products

Pharmaceutical/Biopharmaceutical Products

Product Quality and Biotechnology

Introduction to Product Quality Systems

Biotechnology and the Regulation of Food and Medical Products

Documentation, the Foundation of Quality

Quality Systems in the Production Facility

Quality Systems in the Laboratory

Safety in the Laboratory

Introduction to a Safe Workplace

Working Safely in the Laboratory: General Considerations and Physical Hazards

Working Safely with Chemicals

Working Safely with Biological Materials

Math in the Biotechnology Laboratory: An Overview

Basic Math Techniques

Proportional Relationships

Relationships and Graphing

Descriptions of Data (Descriptive Statistics)

Laboratory Measurements

Introduction to Quality Laboratory Measurements, Tests and Assays

Introduction to Instrumental Methods and Electricity

The Measurement of Weight

The Measurement of Volume

The Measurement of Temperature

The Measurement of pH, Selected Ions and Conductivity

Measurements Involving Light A

Basic Principles and Instrumentation

Quality Assays and Tests

Introduction to Quality Laboratory Tests and Assays

Measurements Involving Light B

Applications and Methods

Laboratory Solutions

Preparation of Laboratory Solutions A: Concentration Expressions and Calculations

Preparation of Laboratory Solutions B: Basic Procedures and Practical Information

Solutions: Associated Procedures and Information

Laboratory Solutions to Support the Activity of Biological Macromolecules

Culture Media for Intact Cells

Basic Separation Methods

Introduction to Filtration

Introduction to Centrifugation

Introduction to Bioseparations

Computers in the Laboratory

Computers: An Overview

Data Handling with Computers

Applications of the Internet to Biotechnology

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