Benson's Microbiological... | Buy

The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Microbiological Applications: A Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, Benson assumes no prior organic chemistry course has been taken.

Laboratory Protocol

Safety

1 Microscopy

1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Fluorescence Microscopy

5 Microscopic Measurements

2 Survey of Microorganisms

6 Protozoans, Algae, and Cyanobacteria

7 Ubiquity of Bacteria

8 The Fungi: Yeasts and Molds

3 Manipulation of Microorganisms

9 Aseptic Technique

10 Pure Culture Techniques

4 Staining and Observation of Microorganisms

11 Smear Preparation

12 Simple Staining

13 Negative Staining

14 Capsular Staining

15 Gram Staining

16 Spore Staining: Two Methods

17 Acid-Fast Staining: Ziehl-Neelsen Method

18 Motility Determination

5 Culture Methods

19 Culture Media Preparation

20 Preparation of Stock Cultures

21 Cultivation of Anaerobes

22 Enumeration of Bacteria: The Standard Plate Count

23 Slime Mold Culture

24 Slide Culture: Molds

6 Bacterial Viruses

25 Determination of a Bacteriophage Titer

26 Burst Size Determination: A One-Step Growth Curve

27 Isolation of Phage from Flies

28 Phage Typing

7 Environmental Influences and Control of Microbial Growth

29 Temperature: Effects on Growth

30 Temperature: Lethal Effects

31 pH and Microbial Growth

32 Water Activity and Osmotic Pressure

33 Ultraviolet Light: Lethal Effects

34 Oligodynamic Action

35 Evaluation of Alcohol: Its Effectiveness as an Antiseptic

36 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

37 Evaluation of Antiseptics: The Filter Paper Disk Method

38 Effectiveness of Hand Scrubbing

8 Identification of Unknown Bacteria

39 Morphological Study of Unknown Bacterium

40 Cultural Characteristics

41 Physiological Characteristics: Oxidation and Fermentation Tests

42 Physiological Characteristics: Hydrolytic Reactions

43 Physiological Characteristics: Biochemical Tests

44 Use of Bergey's Manual

9 Miniaturized Multitest Systems

45 Enterobacteriaceae Identification: The API 20E System

46 Enterobacteriaceae Identification: The Enterotube II System

47 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System

48 Staphylococcus Identification: The API Staph-Ident System

10 Diversity and Environmental Microbiology

49 Isolation of an Antibiotic Producer: The Actinomyces

50 The Nitrogen Cycle: Amonification

51 Symbiotic Nitrogen Fixation: Rhizobium

52 Free-living Nitrogen Fixation: Azotobacter

53 Denitrifcation: Paracoccus denitrificans

54 The Winogradsky Column

55 Purple Nonsulphur Photosynthetic Bacteria

56 Sulfate Reducing Bacteria: Desulfovibrio

57 Bacterial Commensalism

58 Bacterial Synergism

59 Microbial Antagonism

11 Applied Microbiology

60 Bacterial Counts of Foods

61 Bacterial Examination of Water: Qualitative Tests

62 The Membrane Filter Method

63 Reductase Test

64 Microbial Spoilage of Canned Food

65 Microbiology of Alcohol Fermentation

12 Bacterial Genetics and Biotechnology

66 Mutant Isolation by Replica Plating

67 Bacterial Transformation

68 Polymerase Chain Reaction for Amplifying DNA

69 Plasmid Isolation

13 Medical Microbiology and Immunology

70 The Staphylococci: Isolation and Identification

71 The Streptococci: Isolation and Identification

72 Gram Negative Intestinal Pathogens

73 Slide Agglutination Test: Serological Typing

74 Slide Agglutination (Latex) Test: For S. aureus Identification

75 Tube Agglutination Test: The Heterophile Antibody Test

76 White Blood Cell Study: The Differential WBC Count

77 Blood Grouping

78 A Synthetic Epidemic

Appendixes

A Tables

B Indicators, Stains, Reagents

C Media

D Identification Charts

E The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics

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