Bioconjugate Techniques

by Hermanson, Greg T.

• ISBN13: 9780123423351
• ISBN10: 012342335X
• Format: Hardcover
• Copyright: 1996-01-01
• Publisher: Academic Pr

Summary

Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules.

Table of Contents

Bioconjugate Chemistry: Functional Targets

The Chemistry of Reactive Groups

Bioconjugate Reagents: Zero-Length Cross-linkers

Homobifunctional Cross-linkers

Heterobifunctional Cross-linkers

Trifunctional Cross-linkers

Cleavable Reagent Systems

Tags and Probes

Bioconjugate Applications: Preparation of Hapten-Carrier Immunogen Conjugates

Antibody Modification and Conjugation

Immunotoxin Conjugation Techniques

Preparation of Liposome Conjugates and Derivatives

Avidin-Biotin Systems

Preparation of Colloidal-Gold-Labeled Proteins

Modification with Synthetic Polymers

Enzyme Modification and Conjugation

Nucleic Acid and Oligonucleotide Modification and Conjugation

Subject Index

Table of Contents provided by Publisher. All Rights Reserved.