Bioenergetics A Practical Approach

by Brown, Guy C.; Cooper, Chris E.

• ISBN13: 9780199634880
• ISBN10: 0199634882
• Format: Paperback
• Copyright: 1995-10-19
• Publisher: IRL Press

Summary

Bioenergetics is a topic which impinges on a wide range of fundamental problems in biology. The movement of electrons and ions across and through membranes is important in fields such as energy transduction, cell biology, and cell signalling. The maturation of bioenergetics has led to cross-fertilization with other fields and this has led to both the necessity for scientists in other areas, such as clinical scientists, to learn the classical bioenergetic techniques, and the application of foreign techniques to bioenergetics, like molecular biology and electrophysiology. This book describes the core bioenergetic techniques required in the analysis of membrane-translocating events. Topics covered include the measurement of the main fluxes and stoichiometries, membrane transport, redox states and potentials, the isolation of bioenergetic preparations for mitochondria and mitochondrial subcomponents and chloroplast subcomponents, and the reconstitution and characterization of proteins in proteoliposomes. Both established and new techniques are fully described. The book will be a valuable guide for research scientists working in the areas of mammalian and plant biochemistry and cell biology.

Table of Contents

Oxygen, proton, and phosphate fluxes and stoichiometries

Membrane permeability and transport

The protonmotive force

Reconstitution of bioenergetic proteins and the uses of proteoliposomes

Redox states and potentials

Control and regulation in bioenergetics

Patch clamping of bioenergetic membranes and proteoliposomes

Studies of cellular energetics using 31P-NMR

Isolation and characterization of photosynthetic reaction centres from eukaryotic organisms

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