Bioenergetics A Practical Approach

by Brown, Guy C.; Cooper, Chris E.

• ISBN13: 9780199634897
• ISBN10: 0199634890
• Format: Hardcover
• Copyright: 1995-10-19
• Publisher: Oxford University Press

Summary

Bioenergetics is the study of energy in living organisms and is a centraltopic of modern biology. The movement of electrons and ions across and throughmembranes is important in fields such as energy transduction, cell biology andcell signalling. Current research in Bioenergetics impinges on a wide range offundamental problems in biology and scientists in other areas have had to learnclassical bioenergetic techniques. Written by international experts in thefield, this handbook describes all the major techniques and methods of analysisin a practical and accessible format.Contents List: Oxygen, proton, and phosphate fluxes and stoichiometries;Membrane permeability and transport; The protonmotive force; Reconstitution ofbioenergetic proteins and the uses of proteoliposomes; Redox states andpotentials; Control and regulation in bioenergetics; Patch clamping ofbioenergetic membranes and proteoliposomes; Studies of cellular energetics using31P-NMR; Isolation and characterization of photosynthetic reaction centres fromeukaryotic organisms

Table of Contents

Oxygen, proton, and phosphate fluxes and stoichiometries

Membrane permeability and transport

The protonmotive force

Reconstitution of bioenergetic proteins and the uses of proteoliposomes

Redox states and potentials

Control and regulation in bioenergetics

Patch clamping of bioenergetic membranes and proteoliposomes

Studies of cellular energetics using 31P-NMR

Isolation and characterization of photosynthetic reaction centres from eukaryotic organisms

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