Biomolecular Nmr Spectroscopy

by Evans, Jeremy N. S.

• ISBN13: 9780198547662
• ISBN10: 0198547668
• Format: Paperback
• Copyright: 1995-09-28
• Publisher: Oxford University Press

Summary

The technique of nuclear magnetic resonance (NMR) spectroscopy is an important tool in biochemistry and biophysics for the understanding of the structure and ultimately, the function of biomolecules. This textbook explains the salient features of biological NMR spectroscopy to undergraduatesand postgraduates taking courses in NMR, biological NMR, physical biochemistry, and biophysics. Unlike other books in the general field of NMR (except the advanced treatises), the approach here is to introduce and make use of quantum mechanical product operators as well as the classical vectormethod of explaining the bewildering array of pulse sequences available today. The book covers two- dimensional, three- dimensional, and four- dimensional NMR and their application to protein and DNA structure determination. A unique feature is the coverage of the biological aspects of solid- stateNMR spectroscopy. The author provides many selected examples from the research literature, illustrating the applications of NMR spectroscopy to biological proteins.

Table of Contents

Theory

Introduction

Methods for spectral assignment - multidimensional NMR

Obtaining NMR structures

Proteins

Protein structure

Protein folding

Enzymes

Enzyme function

Acyl and phosphoryl transfer enzymes

Other enzymes

Nucleic Acids and Carbohydrates

Nucleic acids and carbohydrates

Membranes

Structure of membranes and membrane proteins

Examples of product operator calculations

Useful NMR data on amino acid residues

Useful NMR data on oligonucleotide residues

Useful NMR data on common solvents

Index

Table of Contents provided by Publisher. All Rights Reserved.