Breast Cancer Research Protocols

by Brooks, Susan A.; Harris, Adrian

• ISBN13: 9781588291912
• ISBN10: 158829191X
• Format: Hardcover
• Copyright: 2006-01-01
• Publisher: Humana Pr Inc

Summary

A collection of both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes.

Table of Contents

Preface	v
Contributors	xi
Part I Preparation of Cell and Tissue Samples
Breast Tissue Banking: Collection, Handling, Storage, and Release of Tissue for Breast Cancer Research	3	(22)
Linda Snell
Peter H. Watson
Frozen Biopsy Collection and Storage: Frozen Biopsy Samples	25	(4)
Russell Leek
Serial Biopsies/Fine-Needle Aspirates and Their Assessment	29	(14)
Irene Boeddinghaus
Stephen R. D. Johnson
Breast Tissue Microarrays	43	(8)
Robert J. Springall
Cheryl E. Gillett
Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA	51	(4)
Naomi Robertson
Russell Leek
Isolation of RNA From Tumor Samples: Single-Step Guanidinium Acid-Phenol Method	55	(6)
Naomi Robertson
Russell Leek
Isolation of DNA From Tumor Samples	61	(4)
Naomi Robertson
Russell Leek
Laser-Assisted Microdissection and Isolation of DNA and RNA	65	(14)
Ulrich Lehmann
Hans Kreipe
Part II Markers of Clinical Outcome and Prognosis
Traditional and Established Indicators of Prognosis and Treatment Success	79	(12)
Derek E. Roskell
Ian D. Buley
Sentinel Lymph Node Biopsy in Breast Cancer	91	(22)
Celia Chao
Kelly M. McMasters
Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer	113	(14)
Michael Z. Gilcrease
Aysegul Sahin
Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections	127	(20)
Bharat Jasani
Anthony Douglas-Jones
Anthony Rhodes
Susan Wozniak
Peter J. Barrett-Lee
Julia Gee
Robert Nicholson
Markers of Apoptosis	147	(14)
Fazlul H. Sarkar
Yiwei Li
Quantitative Angiogenesis in Breast Cancer	161	(30)
Stephen B. Fox
Part III Analysis of Tumor-Derived Proteins and Antigens
Immunohistochemistry	191	(10)
Cheryl E. Gillett
Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry	201	(16)
Tracey M. Carter
Susan A. Brooks
SDS-PAGE and Western Blotting to Detect Proteins and Glycoproteins of Interest in Breast Cancer Research	217	(14)
Chloe Osborne
Susan A. Brooks
Breast Cancer Proteomics Using Two-Dimensional Electrophoresis: Studying the Breast Cancer Proteome	231	(14)
Miriam V. Dwek
Sarah L. Rawlings
Procedures for the Quantitative Protein Determination of Urokinase and Its Inhibitor, PAI-1, in Human Breast Cancer Tissue Extracts by Elisa	245	(24)
Manfred Schmitt
Alexandra S. Sturmheit
Anita Welk
Christel Schnelldorfer
Nadia Harbeck
Part IV Analysis of Genes and Gene Expression in Tumor Specimens
Fluorescence In Situ Hybridization and Comparative Genomic Hybridization	269	(28)
Patricia Gorman
Rebecca Roylance
Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues	297	(12)
Jan K. Blancato
Mary Steele Williams
Robert B. Dickson
Detection of HER2 Gene Amplification by Fluorescence In Situ Hybridization in Breast Cancer	309	(14)
John M. S. Bartlett
Amanda Forsyth
In Situ Hybridization Combined With Immunohistochemistry to Localize Gene Expression	323	(24)
Rosemary Jeffery
Toby Hunt
Richard Poulsom
Quantitation of RNA by Ribonuclease Protection Assay	347	(16)
John W. Moore
Identification of Steroid Hormone-Regulated Genes in Breast Cancer	363	(26)
Bruce R. Westley
Felicity E. B. May
Sequencing of the Tumor Suppressor Gene TP 53	389	(14)
Barbro Linderholm
Torbjorn Norberg
Jonas Bergh
Expression Profiling Using cDNA Microarrays	403	(12)
Chris Jones
Peter Simpson
Alan Mackay
Sunil R. Lakhani
Gene Expression Analysis Using Filter cDNA Microarrays	415	(12)
Peter Simpson
Chris Jones
Alan Mackay
Sunil R. Lakhani
Part V Studying Cancer Cell Behavior In Vitro and In Vivo
Methods to Analyze the Effects of the Urokinase System on Cancer Cell Adhesion, Proliferation, Migration, and Signal Transduction Events	427	(14)
Ute Reuning
Manfred Schmitt
Birgit Luber
Veronika Beck
Viktor Magdolen
Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage	441	(12)
Kum Kum Khanna
Magtouf Gatei
Gordon Tribbick
Models of Hormone Resistance In Vitro and In Vivo	453	(12)
Jennifer MacGregor Schafer
V. Craig Jordan
Generation of Genetically Modified Embryonic Stem Cells for the Development of Knockout Mouse Animal Model Systems	465	(14)
Stephen D. Robinson
Stephen Wilson
Kairbaan M. Hodivala-Dilke
In Vivo Xenograft Models of Breast Cancer Metastasis	479	(10)
Ursula Valentiner
Susan A. Brooks
Udo Schumacher
Neoadjuvant Endocrine Therapy Models	489	(14)
Juliette Murray
William R. Miller
J. Michael Dixon
Primary Mouse Endothelial Cell Culture for Assays of Angiogenesis	503	(8)
Louise E. Reynolds
Kairbaan M. Hodivala-Dilke
Index	511