Calcium Measurement Methods

by Verkhratsky, Alexei; Petersen, Ole H.

• ISBN13: 9781607614753
• ISBN10: 1607614758
• Edition: 1st
• Format: Hardcover
• Copyright: 2009-11-30
• Publisher: Humana Pr Inc

Summary

Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signalling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In Calcium Measurement Methods, a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful Neuromethods'„¢ series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field.Cutting-edge and concise, Calcium Measurement Methods serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.

Table of Contents

Preface to the Series	p. v
Preface	p. vii
Contributors	p. xi
Principles of the Ca2+ Homeostatic/Signalling System	p. 1
Ca2+ Recordings: Hardware and Software (From Microscopes to Cameras)	p. 13
Ca2+ Imaging: Principles of Analysis and Enhancement	p. 57
Bioluminescent Ca2+ Indicators	p. 81
Monitoring Calcium Levels With Genetically Encoded Indicators	p. 101
Intracellular Calcium-Sensitive Microelectrodes	p. 119
Ca2+ Caging and Uncaging	p. 127
Ca2+ Imaging of Intracellular Organelles: Endoplasmic Reticulum	p. 147
Ca2+ Imaging of Intracellular Organelles: Mitochondria	p. 169
Ca2+ Imaging of Dendrites and Spines	p. 189
In Vivo 2+ Imaging of the Living Brain Using Multi-cell Bolus Loading Technique	p. 205
Ca2+ Imaging of Glia	p. 221
Index	p. 251
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