Cardiovascular Physiology in the Genetically Engineered Mouse

by Hoit, Brian D., M.D.; Walsh, Richard A., M.D.

• ISBN13: 9780792375364
• ISBN10: 079237536X
• Edition: 2nd
• Format: Hardcover
• Copyright: 2001-10-01
• Publisher: Kluwer Academic Pub

Summary

The enormous advances in molecular biology and genetics coupled with the progress in instrumentation and surgical techniques have produced a voluminous and often bewildering quantity of data. The primary objective of a second edition of Cardiovascular Physiology in the Genetically Engineered Mouse is to interpret critically the literature and to provide a framework for the enormous amount of information in this burgeoning field. As in the first edition, the monograph serves as a practical guide for the investigator interested in the functional methods used to characterize the murine cardiovascular phenotype. The monograph is organized into three parts. The first deals with principles of transgenesis and homologous recombination. The second part, which again is the largest, discusses the various techniques used to assess the cardiovascular mechanical, metabolic, and electrophysiologic phenotype. This section is organized in a hierarchical manner- i.e. from isolated myocyte to isolated heart to the intact, anesthetized and conscious mouse. The third part examines techniques used to evaluate murine smooth muscle function, genetic mouse models of hypertrophy and heart failure, and the methods to assess the cardiovascular phenotype in the developing mouse embryo. In addition, newer methods that 'push the envelope', such as magnetic resonance imaging (MRI), positron emission tomography (PET), ultrasound biomicroscopy (UBM), and computed tomography (microCT) are discussed. Expanded and updated, each chapter is richly enhanced with original tables and figures, and in many cases, extensively rewritten when compared with the first edition. An essential and enduring goal of this second edition is to continue to facilitate interactions between the basic science disciplines and help bridge the gap between molecular biology and physiology.

Table of Contents

Preface	xiii
PART ONE
The Hierarchical Approach to Analysis of Cardiovascular Functions in Mice: An Overview	1	(16)
Richard A. Walsh
Genetic Alterations and Modeling of Cardiovascular Physiology	17	(22)
Albrecht Schmidt
Vivek J. Kadambi
Karen B. Young
Evangelia G. Kranias
Conditional Transgenesis	39	(14)
Tetsuo Minamino
Michael D. Schneider
Array Transcription Profiling: Molecular Phenotyping of Rodent Cardiovascular Models	53	(10)
Susan Acton
Raju Jeyaseelan
Vivek J. Kadambi
Roger E. Breitbart
Murine Cardiovascular Development	63	(8)
Steven A. Fisher
PART TWO
Isolated Myocyte Mechanics and Calcium Transients	71	(20)
Zhi Su
William H. Barry
Single Cell Patch---Clamp Analysis of Mouse Cardiac Myocytes	91	(22)
Nipavan Chiamvinonvat
M. Jane Lalli
Atsuko Yatani
The Isolated Isovolumic (Langendorff) Heart Preparation	113	(16)
Martin LeWinter
The Isolated Work-Performing and Ejecting Mouse Heart Preparation, Comparison and Quantification of Cardiac Performance in Transgenic and Wild-type Mice	129	(22)
Ingrid L. Grupp
Jo El Schultz
George Sfyris
Gunter Grupp
31P NMR Spectroscopy of the Mouse Heart	151	(14)
Joanne S. Ingwall
Maryan M. Javadpour
Wenfeng Miao
Electrophysiological Characterization of the Mouse Heart Using Voltage-Sensitive Dyes	165	(12)
Benjamin C. Eloff
David Rosenbaum
Echocardiographic Assessment of the Mouse Heart and Aorta	177	(14)
Brian D. Hoit
In Vivo Invasive Hemodynamic Studies in Mice	191	(16)
Brian D. Hoit
Pressure-Volume Relations	207	(16)
Dimitrios Georgakopoulos
David A. Kass
Assessment of Left Ventricular Diastolic Function in Mice	223	(14)
Michael R. Zile
Anne Hauef
Elizabeth Hapke
B.S. Janet Simsic
In Vivo Electrophysiologic Studies in Mice	237	(20)
Charles I. Berul
Paul J. Yang
Cardiovascular Physiology in Mice: Conscious Function Measurements and Effects of Anesthesia	257	(20)
Stephen Vatner
Gen. Takagi
Kuniya Asai
Richard P. Shannon
Exercise and Oxygen Consumption in the Mouse	277	(26)
Kavin H. Desai
Daniel Bernstein
Clinical Laboratory Analysis of the Genetically Manipulated Mouse	303	(16)
William Lewis
PART THREE
Smooth Muscle Studies Using Gene-Altered Mouse Models: A Users' Guide	319	(16)
Roy L. Sutliff
Richard J. Paul
Models of Cardiac Disease in the Mouse, Cardiac Hypertrophy and Heart Failure	335	(18)
Yasuhiro Ikeda
John Ross, Jr.
Analysis of Murine Embryonic Cardiovascular Phenotype	353	(24)
Kimimasa Tobita
Joseph P. Tinney
Bradley B. Keller
Newer In Vivo Imaging Modaltities	377	(20)
Roee S. Lazebnik
David L. Wilson
Index	397