Cell Culture Engineering VI

by Betenbaugh, Michael J.; Chalmers, Jeffrey J.; Arathoon, Rob; Chaplen, Frank W. R.

• ISBN13: 9780792355755
• ISBN10: 079235575X
• Format: Hardcover
• Copyright: 1999-06-01
• Publisher: Kluwer Academic Pub

Summary

The latest edition in this continuing series includes the newest advances in the rapidly evolving field of animal cell culture, genetic manipulations for heterologous gene expression, cell line enhancements, improved bioreactor designs and separations, gene therapy manufacturing, tissue engineering, anti-apoptosis strategies and cell cycle research. The contents include new research articles as well as critical reviews on emerging topics such as viral and viral-like agent contamination of animal cell culture components. These papers were carefully selected from contributions by leading academic and industrial experts in the biotechnology community at the recent Cell Culture Engineering VI Meeting in San Diego, USA, 1998. However, the book is not merely a proceedings. Audience: Biochemical engineers, cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment and the biotechnology or pharmaceutical industry.

Table of Contents

Cell therapy in kidney failure	1	(8)
H.D. Hunes
A.J. Funke
D.A. Buffington
Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE)	9	(10)
T.L. Aldrich
J.N. Thomas
A.E. Morris
Effects on growth behavior in continuous hybridoma cell cultures The role of viral contamination	19	(12)
A. Hawerkamp
D. Lutkemeyer
F. Gudermann
A. Falkenhain
H. Buntemeyer
J. Lehmann
Isolation, characterization and recombinant protein expression in Veggie-CHO A serum-free CHO host cell line	31	(12)
B. Rasmussen
R. Davis
J. Thomas
P. Reddy
Collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them	43	(6)
S.J. Wessmanb
R.L. Levings
An overview of viral and viral-like agents in cell culture systems	49	(4)
J.C. Petricciani
New adenovirus vectors for protein production and gene transfer	53	(12)
B. Massie
D.D. Mosser
M. Koutroumanis
I. Vitte-Mony
L. Lamoureux
F. Couture
L. Paquet
C. Guilbault
J. Dionne
D. Chahla
P. Jolicoeur
Y. Langelier
Modulation of cell cycle progression and of antibody production in mouse hybridomas by a nucleotide analogue	65	(8)
F. Franek
A. Holy
I. Votruba
T. Eckschlager
Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth---associated production of a foreign protein, β-galactosidase	73	(8)
F.W.F. Lee
C.B. Elias
P. Todd
D.S. Kompala
A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy	81	(10)
G. Schoofs
T.J. Monica
J. Ayala
J. Horwitz
T. Montgomery
G. Roth
F.J. Castillo
Recombinant insulin-like growth factor-I (IGF-I) production in Super-CHO results in the expression of IGF-I receptor and IGF binding protein 3	91	(10)
N-A. Sunstrom
M. Baig
L. Cheng
D.P. Sugyiono
P. Gray
Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions	101	(10)
J. Varani
F. Piel
S. Josephs
T.F. Beals
W.J. Hillegas
Regulated multicistronic expression technology for mammalian metabolic engineering	111	(16)
M. Fussenegger
S. Moser
J.E. Bailey
Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions	127	(12)
A. De Leon
H. Mayani
O.T. Ramirez
Historical reflections on cell culture engineering	139	(8)
A.S. Lubiniecki
Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation	147	(10)
J.H. Parham
M.A. Iannone
L.K. Overton
J.T. Hutchins
Population balance model of in vivo neutrophil formation following bone marrow rescue therapy	157	(6)
L.K. Nielsen
J.G. Bender
W.M. Miller
E.T. Papoutsakis
Mammalian cell retention devides for stirred perfusion bioreactors	163	(14)
S.M. Woodside
B.D. Bowen
J.M. Piret
Variable functions of bcl-2 in mediating bioreactor stress-induced apoptosis in hybridoma cells	177	(12)
A. Perani
R. P. Singh
R. Chauhan
M. Al-Rubeai
Apoptosis-resistant NS/0 E1B-19K myelomas exhibit increased viability and chimeric antibody productivity under cell cycle modulating conditions	189	(16)
S. Mercille
B. Massie
Effects of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures	205	(8)
E-J. Schlaeger
K. Lundstrom
Effects of CO2 and osmolality on hybridoma cells growth, metabolism and monoclonal antibody production	213	(16)
V.M. deZengotita
R. Kimura
W.M. Miller
pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons	229
M. Fussenegger
S. Moser
J.E. Bailey