Cell Fusion

by Chen, Elizabeth H.

• ISBN13: 9781588299116
• ISBN10: 1588299112
• Edition: 1st
• Format: Hardcover
• Copyright: 2008-09-03
• Publisher: Humana Pr Inc
• More Book Details

Summary

Exciting work in the past decade has revealed commonalities and differences among individual cell fusion events. In Cell Fusion: Overviews and Methods, a team of leading experts provide a collection of overviews that outline our current understanding of cell fusion and methods that present classic and state-of-the-art experimental approaches in a variety of systems. Divided into two convenient parts, the volume begins with nine overviews that describe different cell fusion events in models from yeast to mammals, and it continues with thirteen chapters illustrating commonly used methods to assay cell fusion in particular systems. As a part of the highly successful Methods in Molecular Biology series, these methods chapters compile step-by-step, readily reproducible protocols with lists of the necessary materials and reagents, along with tips on troubleshooting and avoiding known pitfalls. Cutting-edge and user-friendly, Cell Fusion: Overviews and Methods serves as a comprehensive resource for anyone, expert or novice, interested in the fascinating biological process of cell fusion. Book jacket.

Table of Contents

Preface	p. v
Contributors	p. ix
CEll Fusion
Yeast Mating: A Model System for Studying Cell and Nuclear Fusion	p. 3
Cell Fusion in the Filamentous Fungus, Neurospora crassa	p. 21
Gametic Cell Adhesion and Fusion in the Unicellular Alga Chlamydomonas	p. 39
Cell Fusion in Caenorhabditis elegans	p. 53
Myoblast Fusion in Drosophila	p. 75
Mammalian Fertilization Is Dependent on Multiple Membrane Fusion Events	p. 99
Molecular Control of Mammalian Myoblast Fusion	p. 115
Placenta Trophoblast Fusion	p. 135
Macrophage Fusion: Molecular Mechanisms	p. 149
Cell Fusion Assays
Cell Fusion Assays for Yeast Mating Pairs	p. 165
Ultrastructural Analysis of Cell Fusion in Yeast	p. 197
Isolation and In Vitro Binding of Mating Type Plus Fertilization Tubules From Chlamydomonas	p. 213
Optical Imaging of Cell Fusion and Fusion Proteins in Caenorhabditis elegans	p. 223
Ultrastructural Imaging of Cell Fusion in Caenorhabditis elegans	p. 245
Live Imaging of Drosophila Myoblast Fusion	p. 263
Ultrastructural Analysis of Myoblast Fusion in Drosophila	p. 275
A Genomic Approach to Myoblast Fusion in Drosophila	p. 299
Mesenchymal Cell Fusion in the Sea Urchin Embryo	p. 315
Sperm-Egg Fusion Assay in Mammals	p. 335
Quantitative Assays for Cell Fusion	p. 347
Fusion Assays and Models for the Trophoblast	p. 363
Methods to Fuse Macrophages In Vitro	p. 383
Index	p. 397
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