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Preface | p. v |
Contributors | p. ix |
Cell-Free Protein Preparation Through Prokaryotic Transcription-Translation Methods | p. 1 |
PURE Technology | p. 11 |
The Cell-Free Protein Synthesis System from Wheat Germ | p. 23 |
A Cell-Free Protein Synthesis System from Insect Cells | p. 31 |
Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells | p. 43 |
Analysis of Protein Functions Through a Bacterial Cell-Free Protein Expression System | p. 53 |
Cell-Free-Based Protein Microarray Technology Using Agarose/DNA Microplate | p. 63 |
An Efficient Approach to the Production of Vaccines Against the Malaria Parasite | p. 73 |
Protein Engineering Accelerated by Cell-Free Technology | p. 85 |
Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds | p. 101 |
NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System | p. 113 |
Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination | p. 127 |
Cell-Free Protein Synthesis for Structure Determination by X-ray Crystallography | p. 149 |
Production of Multi-Subunit Complexes on Liposome Through an E. coli Cell-Free Expression System | p. 161 |
Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System | p. 173 |
Strategies for the Cell-Free Expression of Membrane Proteins | p. 187 |
Production of Membrane Proteins Through the Wheat-Germ Cell-Free Technology | p. 213 |
Ribosome Display with the PURE Technology | p. 219 |
Incorporation of 3-Azidotyrosine into Proteins Through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification | p. 227 |
Synthesis of Functional Proteins Within Liposomes | p. 243 |
Index | p. 257 |
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