Chemokine Protocols

by Proudfoot, Amanda E. I.; Wells, Timothy N. C.; Power, Christine A.

• ISBN13: 9780896037229
• ISBN10: 0896037223
• Format: Hardcover
• Copyright: 2000-04-01
• Publisher: Humana Pr Inc

Summary

Expert investigators describe in detail the most widely used techniques in chemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience. Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today's gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins.

Table of Contents

Preface	v
Contributors	xi
Purification of Chemokines from Natural Sources	1	(10)
Jens-M. Schroder
Cloning of Novel Chemokines Using a Signal Sequence Trap Method	11	(12)
Toshio Imai
Chemokine Expression in Insect Cells	23	(10)
Toshio Imai
Expression of Chemokines in Escherichia coli	33	(8)
Michael D. Edgerton
Lars-Ole Gerlach
Thomas P. Boesen
Bernard Allet
Expression of Chemokines in the Periplasmic Space of E. coli	41	(6)
Jochen Pfirstinger
Matthias Mack
Synthesis of Chemokines	47	(18)
Ian Clark-Lewis
Identification of Novel Chemokines from Expressed Sequence Tag Databases	65	(10)
Timothy N. C. Wells
Manuel C. Peitsch
Purification of Recombinant Chemokines from E. coli	75	(14)
Amanda E. I. Proudfoot
Frederic Borlat
Chemokine Receptor Cloning	89	(10)
Philip M. Murphy
Generation of Stable Cell Lines Expressing Chemokine Receptors	99	(6)
Christine A. Power
Alexandra Meyer
Modified Microchemotaxis Assays	105	(8)
Dennis D. Taub
Transwell Chemotaxis	113	(8)
Paul D. Ponath
Juan Wang
Heidi Heath
Endothelial Cell Chemotaxis Assays	121	(8)
Darcey Black
Radiolabeled Chemokine Binding Assays	129	(6)
Bruce L. Daugherty
Salvatore J. Siciliano
Martin S. Springer
Scintillation Proximity Binding Assay	135	(8)
Sami Alouani
Calcium Mobilization	143	(6)
Raphaele Buser
Amanda E. I. Proudfoot
Actin Polymerization	149	(4)
Jorn Elsner
Alexander Kapp
Reactive Oxygen Release	153	(4)
Jorn Elsner
Alexander Kapp
Histamine Release	157	(6)
Michael A. Lett-Brown
Rafeul Alam
Measurement of Phosphoinositide 3-Kinase Activity	163	(10)
Stephen G. Ward
Glycosaminoglycan Binding Assays	173	(6)
Arlene J. Hoogewerf
Gabriele S. V. Kuschert
CFU-A Assay for Measurement of the Antiproliferative Effects of Chemokines on Murine Early Hemopoietic Progenitors	179	(12)
Gerard J. Graham
Mary G. Freshney
Downmodulation and Recycling of Chemokine Receptors	191	(6)
Matthias Mack
Detlef Schlondorff
Analysis of Chemokine Receptor Endocytosis and Recycling	197	(12)
Nathalie Signoret
Mark Marsh
Chemokine Inhibition of HIV Infection	209	(14)
Jacqueline D. Reeves
Graham Simmons
The Production of Chemokine Specific Monoclonal Antibodies: Chemokine RANTES	223	(8)
Peter J. Nelson
Monoclonal Antibodies to Chemokine Receptors	231	(12)
Paul D. Ponath
Nasim Kassam
Shixin Qin
Targeted Expression of Chemokines In Vivo	243	(10)
Iqbal Grewal
Long Gu
Susan Tseng
Barrett J. Rollins
Chemokine Knockout Mice	253	(6)
Marc E. Rothenberg
Chemokine Receptor Knockout Mice	259	(16)
Ji-Liang Gao
Philip M. Murphy
Measurement of Eosinophil Accumulation In Vivo	275	(10)
Maria-Jesus Sanz
Peter J. Jose
Timothy J. Williams
Murine Model of Allergic Lung Inflammation	285	(10)
Sami Alouani
Pierre Juillard
Yolande Chvatchko
Murine Models of Airway Inflammation	295	(8)
Emma M. Campbell
Nicholas W. Lukacs
Rat Models of Respiratory Inflammation	303	(8)
Andrew S. McWilliam
Patrick G. Holt
Murine Model of Crescentic Nephritis	311	(8)
Clare Lloyd
Jose-Carlos Gutierrez-Ramos
Rabbit Models of Pneumonia, Peritoneal Sepsis, and Lung Injury	319	(12)
Charles W. Frevert
Gustavo Matute-Bello
Thomas R. Martin
Index	331