Chromatographic Methods in Clinical Chemistry and Toxicology

by Bertholf, Roger; Winecker, Ruth

• ISBN13: 9780470023099
• ISBN10: 0470023090
• Edition: 1st
• Format: Hardcover
• Copyright: 2007-02-27
• Publisher: WILEY

Summary

This book aims to fill the gap that exists between theoretical treatments of chromatography, and clinical chemistry and toxicology texts, which focus almost exclusively on clinical relevance and applications. Chromatography has a vast array of clinical applications, and though the chromatographic methods were first introduced decades ago, new applications of this technology are being used to explore previously inaccessible frontiers in clinical diagnostics and toxicological testing. An up-to-date book devoted to clinical and toxicological applications of chromatographic methods will serve as an instructional and reference text, useful to students, laboratory technicians, and researchers.

Author Biography

Professor R.L. Bertholf, Associate Professor and Director of Clinical Chemistry and Toxicology, Department of Pathology, University of Florida Health Science Center, Jacksonville, FL, USA.

Dr R.E. Winecker, Chief Toxicologist, North Carolina Office of the Chief Medical Examiner.

Table of Contents

Preface

List of Contributors

Quality Assurance, Quality Control and Method Validation in Chromatographic Applications

Introduction

History

Definition of Quality Assurance and Quality Control

Professional Organizations

Internal Quality Assurance and Control

Standard operating procedure manual

Method development

Method validation

Accuracy

Precision

Recovery

Lower limits of detection (sensitivity) and quantitation

Range of linearity

Specificity

Stability

Carryover

Ruggedness

Selection of a reference standard

Selection of an internal standard and standard addition

Selection of derivatization agent

Selection of ions for selected-ion monitoring or full-scan analysis

Chromatographic performance

Statistical evaluation of quality control

External Quality Assurance

References

Liquid Chromatographic-Mass Spectrometric Measurement of Anabolic Steroids

Introduction

LC-MS Analysis of Synthetic Steroids or Animal Samples

LC-MS Analysis of Natural Androgens in Human Samples

Conclusion

References

High-performance Liquid Chromatography in the Analysis of Active Ingredients in Herbal Nutritional Supplements

Introduction

St John's Wort

Drug interactions with St John's wort

Measurement of active ingredients of St John's wort using HPLC

Analysis of St John's wort extract with other analytical techniques

Measurement of hypericin and hyperforin in human plasma using HPLC

Herbal Supplements with Digoxin-like Immunoreactivity

Use of HPLC for the determination of chan su, danshen and ginsengs

Herbal Remedies and Abnormal Liver Function Tests

Use of GC-MS and HPLC for the measurement of active components

Ginkgo Biloba

Analysis of components of ginkgo biloba by HPLC

Echinacea

Analysis of active components of echinacea by HPLC

Valerian

Analysis of components of valerian by HPLC

Feverfew

Analysis of parthenolide by HPLC

Garlic

Measurement of components of garlic by HPLC

Ephedra (Ma Huang) and Related Drugs

Analysis of active components of ephedra-containing products

Conclusions

References

Measurement of Plasma L-DOPA and L-Tyrosine by High-Performance Liquid Chromatography as a Tumor Marker in Melanoma

Introduction

Melanogenesis

Overview of the pathway

Potential tumor markers

L-DOPA Alone

Urine analysis

Blood (plasma or serum) analysis

L-DOPA/L-Tyrosine Ratio

Technical aspects

Clinical results

Future directions

Conclusion

References

Hypersensitive Measurement of Proteins by Capillary Isoelectric Focusing and Liquid Chromatography-Mass Spectrometry

Introduction

A Robust CIEF-RPLC Interface

First-Generation CIEF-RPLC-MS System for Proteins

Second-Generation CIEF-RPLC-MS System

Future Improvements

Acknowledgment

References

Chromatographic Measurement of Transferrin Glycoforms for Detecting Alcohol

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