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9780123339270

Colloidal Gold Vol. 1 : Principles, Methods, and Applications

by
  • ISBN13:

    9780123339270

  • ISBN10:

    0123339278

  • Format: Hardcover
  • Copyright: 1989-11-01
  • Publisher: Academic Pr

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Summary

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. Key Features * Among the first books to cover principles and methodology of colloidal gold in microscopy * Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book * Discusses problems and limitations of techniques * Guides users to avoid problems and choose the correct procedures for specific applications * Contributors are eminent authorities in their fields Topics covered in Volume 3 include: * Production of monoclonal and polyclonal antibodies * Lowicryl, polar, and apolar resins * Preserving and localizing antigenicity; sectioning; high-resolution morphological localization * Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation * Applications to biomedical studies * Colloidal gold in conjunction with video-enhanced microscopy

Table of Contents

The Development and Application of Colloidal Gold as a Microscopic Probe. Methods for Synthesis of Colloidal Gold. Protein A
b1Gold and Protein G-
b1Gold Postembedding Immunoelectron Microscopy. Preparation and Application of Lectin
b1Gold Complexes. Colloidal Gold for Multiple Staining. Immunogold Labeling of Ultrathin Cryosections. Colloidal Gold for Scanning Electron Microscopy. Backscattered Electron Imaging of the Colloidal Gold Marker on Cell Surfaces. Silver-Enhanced Colloidal Gold Method. Silver-Enhanced Colloidal Gold for Light Microscopy. Strategies in Colloidal Gold Labeling of Cell Surfaces and Cytoskeletal Elements of Cultured Cells. Colloidal Gold
b1Immunoreplica Method. Immunogold Double-Diffusion Method. Colloidal Gold-Labeled Agarose

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