Colloidal Gold Vol. 1 : Principles, Methods, and Applications

by Hayat, M. A.

• ISBN13: 9780123339294
• ISBN10: 0123339294
• Format: Hardcover
• Copyright: 1991-01-01
• Publisher: Academic Pr

Summary

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. Key Features * Among the first books to cover principles and methodology of colloidal gold in microscopy * Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book * Discusses problems and limitations of techniques * Guides users to avoid problems and choose the correct procedures for specific applications * Contributors are eminent authorities in their fields Topics covered in Volume 3 include: * Production of monoclonal and polyclonal antibodies * Lowicryl, polar, and apolar resins * Preserving and localizing antigenicity; sectioning; high-resolution morphological localization * Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation * Applications to biomedical studies * Colloidal gold in conjunction with video-enhanced microscopy

Table of Contents

Some Basic Concepts for the Choice of Methods

Techniques for the Production of Monoclonal and Polyclonal Antibodies

Lowicryl Resins

Polar and Apolar Lowicryl Resin for Immunolabeling

Comparable Yield of Immunolabel by Resin Sections and Thawed Cryosectins

Colloidal Gold Labeling for Determining Cell Surface Area

Quantitation of Colloidal Gold by Electron Microscopy

Simultaneous Demonstration of Antigens on Outer and Protoplasmic Surfaces of the Plasma Membrane by Replica Immunocytochemistry

Double Labeling of Antigenic Sites on Cell Surfaces Imaged with Backscattered Electrons

Location and Identification of Colloidal Gold Particles with an Energy Dispersive Analyzer

Use of Dinitrophenol IgG Conjugates: Immunogold Labeling of Cellular Antigens on Thin Sections of Osmicated and Epon-Embedded Specimens

Preparation and Application of Insulin-Gold Complex

Microinjection of Colloidal Gold

Combined Diaminobenzidine-Colloidal Gold Staining

Colloidal Gold Techniques and the Immunocytochemistry of Antigen-Antibody Reactions at Interfaces

Colloidal Gold Labeling for Diagnostic Pathology

Immunogold Silver Staining Using Microwave Irradiation

A Correlative Approach to Colloidal Gold Labeling with Video-Enhanced Light Microscopy, Low Voltage Scanning Electron Microscopy, and High Voltage Electron Microscopy

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