A Color Atlas for Human Assisted Reproduction Laboratory and Clinical Insights

by Patrizio, Pasquale; Tucker, Michael J.; Guelman, Vanessa

• ISBN13: 9780781737692
• ISBN10: 0781737699
• Edition: 1st
• Format: Hardcover
• Copyright: 2003-02-20
• Publisher: LWW

Summary

Featuring more than 300 full-color photographs, this atlas is a comprehensive guide to the complex procedures used in assisted reproduction. It shows readers how to utilize the latest technologies and correlates the laboratory and clinical components of assisted reproduction.The book depicts oocytes, embryos, and blastocysts at various stages of division and offers guidelines for assessing oocytes and embryos. The authors give detailed instructions on ovarian stimulation, intracytoplasmic sperm injection, assisted hatching, cryopreservation, extended in vitro culturing, preimplantation genetic diagnosis, and embryo transfer techniques. Close attention is also given to assessment of oocytes and embryos from patients with endometriosis and other pathologies.

Table of Contents

Contributing Authors	ix
Foreword	xi
Luigi Mastroianni, Jr.
Foreword	xiii
Robert G. Edwards
Preface	xv
Acknowledgments	xvi
SECTION I: LABORATORY INSIGHTS
The Biology of Fertilization in Humans	3	(14)
Laura Hewitson
Gerald P. Schatten
The New Imaging Technique Applied to Human Reproduction	17	(8)
Cathy Boutin
Clifford Hoyt
The Oocyte	25	(24)
Vanessa Guelman
Pasquale Patrizio
Fertilization and Cleavage	49	(22)
Vanessa Guelman
Classification of Pronuclei and Polarity of the Zygote: Correlations with Outcome	71	(18)
Lynette Scott
Intracytoplasmic Sperm Injection	89	(10)
Vanessa Guelman
Michael J. Tucker
Morphological Scoring of Human Embryos and Its Relevance to Blastocyst Transfer	99	(10)
Michael J. Tucker
Juergen Liebermann
Assisted Hatching	109	(28)
Marc P. Portmann
Ronald F. Feinberg
Oocyte and Embryo Cryopreservation	137	(24)
Michael J. Tucker
Juergen Liebermann
Practical Aspects of Human Ovarian Tissue Storage	161	(18)
Debra A. Gook
David H. Edgar
Polar Body Biopsy, Embryo Biopsy, and Fixation for Fluorescent In Situ Hybridization Analysis	179	(16)
Santiago Munne
Mireia Sandalinas
Esther Velilla
Peter Nagy
Nuclear and Cytoplasmic Transfer in Mouse and Human Oocytes	195	(14)
John Zhang
Hui Liu
Lewis Krey
Jamie A. Grifo
SECTION II: CLINICAL INSIGHTS
The Clinical Component of Assisted Reproductive Technologies	209	(20)
Pasquale Patrizio
The Technique of Ovarian Transplantation: Laboratory and Clinical Aspects	229	(12)
Kutluk Oktay
Erkan Buyuk
SECTION III: ADDITIONAL TOPICS
Media Used in the Assisted Reproductive Technologies Laboratories	241	(16)
Patrick Quinn
Cryopreservation Protocols	257	(20)
Michael J. Tucker
Paula C. Morton
Juergen Liebermann
Quality Control and Accreditation	277	(20)
Brooks A. Keel
Tammie K. Schalue
The Ethics of Stem Cell Therapy	297	(14)
Glenn McGee
Pasquale Patrizio
Vanessa Kuhn
Claire Robertson-Kraft
Subject Index	311