Computational Analysis of Biochemical Systems: A Practical Guide for Biochemists and Molecular Biologists

by Eberhard O. Voit

• ISBN13: 9780521785792
• ISBN10: 0521785790
• Edition: CD
• Format: Paperback
• Copyright: 2000-09-04
• Publisher: Cambridge University Press

Summary

Throughout the foreseeable future, research in medicine, molecular biology, and biotechnology will be dominated by scientific advances resulting from the Human Genome Project. Knowledge of genomes will elucidate disease patterns and promise improved and personalized treatments. Food and drug development will be revolutionized by focused manipulation of genes and biochemical processes. Researchers will begin to construct metabolic pathways from scratch. A true understanding of genetic and metabolic function and design will depend on mathematical and computational methods for analyzing biochemical systems. It will require new ways of thinking and novel approaches of integrative analysis. This book teaches biochemists and molecular biologists the use of modern computational methods for the analysis of complex biomedical systems without assuming more than a modest background in mathematics. The book begins with representations of biochemical systems, provides guidelines for setting up models, discusses in detail mathematical and computational methods of parameter estimation and model analysis, and ultimately connects to the modern literature with four detailed case studies. Every step is illustrated with examples and explored with accompanying software.

Table of Contents

Preface	ix
Introduction	1	(10)
Graphical Representation of Biochemical Systems	11	(26)
Models of Biochemical Systems	37	(39)
From Maps to Equations	76	(21)
Computer Simulation	97	(46)
Parameter Estimation	143	(50)
Analytical Steady-State Evaluation	193	(29)
Sensitivity Analysis	222	(38)
Anaerobic Fermentation Pathway in Saccharomyces cerevisiae	260	(33)
Diagnosis and Refinement of a Model of the Tricarboxylic Acid Cycle in Dictyostelium discoideum	293	(33)
A Sequence of Models Describing Purine Metabolism	326	(39)
Algebraic Analysis of the Initial Steps of the Glycolytic-Glycogenolytic Pathway in Perfused Rat Liver	365	(34)
Epilogue - Canonical Modeling Beyond Biochemistry	399	(14)
Appendix	413	(30)
Hints and Partial Solutions	443	(26)
References	469	(30)
Author Index	499	(8)
Subject Index	507