Confocal Microscopy

by Abelson; Simon; Conn

• ISBN13: 9780121822088
• ISBN10: 0121822087
• Edition: 1st
• Format: Hardcover
• Copyright: 1999-08-24
• Publisher: Elsevier Science

Summary

This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Table of Contents

CONTRIBUTORS TO VOLUME 307	ix	(4)
PREFACE	xiii	(2)
VOLUMES IN SERIES	xv
Section I. Theory and Practical Considerations	3	(26)
1. Theoretical Basis of Confocal Microscopy	3	(17)
ROBERT H. WEBB
2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope	20	(9)
S. K. KONG
SAMUEL KO
C. Y. LEE
P. Y. LUI
Section II. General Techniques	29	(144)
3. Equipment for Mass Storage and Processing of Data	29	(26)
GUY COX
4. Antifading Agents for Confocal Fluorescence Microscopy	55	(24)
MIGUEL BERRIORS
KIMBERLY A. CONLON
DAVID E. COLFLESH
5. Mounting Techniques for Confocal Microscopy	79	(5)
MANABU KAGAYAMA
YASUYUKI SASANO
6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy	84	(24)
HISASHI HASHIMOTO
HIROSHI ISHIKAWA
MORIAKI KUSAKABE
7. Cytotomography	108	(11)
TORSTEN PORWOL
ANJA-ROSE STROHMAIER
EBERHARD SPIESS
8. Use of Confocal Microscopy to Investigate Cell Structure and Function	119	(16)
GHASSAN BKAILY
DANIELLE JACQUES
PIERRE POTHIER
9. Combining Laser Scanning Confocal Microscopy and Electron Microscopy	135	(17)
XUEJUN SUN
10. Construction of Line-Scan Confocal Microscope for Physiological Recording	152	(21)
NICK CALLAMARAS
IAN PARKER
Section III. Measurement of Subcellular Relations and Volume Determinations	173	(146)
11. Three-Dimensional Visualization of Cytoskeleton by Confocal Laser Scanning Microscopy	173	(17)
WERNER BASCHONG
MARKUS DURRENBERGER
ANNA MANDINOVA
ROSMARIE SUETTERLIN
12. Chromosome Spread for Confocal Microscopy	190	(17)
NADIR M. MARALDI
SILVANO CAPITANI
CATERINA CINTI
LUCA M. NERI
SPARTACO SANTI
STEFANO SQUARZONI
LIBORIO STUPPIA
FRANCESCO A. MANZOLI
13. Visualization of Nuclear Pore Complex and Its Distribution by Confocal Laser Scanning Microscopy	207	(23)
ULRICH KUBITSHCHECK
THORSTEN KUES
REINER PETERS
14. Measurement of Tissue Thickness Using Confocal Microscopy	230	(16)
JAMES V. JESTER
W. MATTHEW PETROLL
H. DWIGHT CAVANAGH
15. Measurements of Vascular Remodeling by Confocal Microscopy	246	(28)
SILVIA M. ARRIBAS
CRAIG J. DALY
JOHN C. McGRATH
16. Cell Volume Measurements by Fluorescence Confocal Microscopy: Theoretical and Practical Aspects	274	(22)
LOTHAR A. BLATTER
17. Volume Measurements in Confocal Microscopy	296	(23)
GARY C. SIECK
CARLOS B. MANTILLA
Y. S. PRAKASH
Section IV. Imaging of Processes	319	(106)
18. Quantitation of Phagocytosis by Confocal Microscopy	319	(9)
GEORGE F. BABCOCK
19. Measurement of Secretion in Confocal Microscopy	328	(12)
AKIHISA SEGAWA
20. Receptor-Ligand Internalization	340	(11)
GUIDO ORLANDINI
NICOLETTA RONDA
RITA GATTI
GIAN CARLO GAZZOLA
ALBERICO BORGHETTI
21. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy	351	(17)
DAVID W. PISTON
SUSAN M. KNOBEL
22. Localization of Proteases and Peptide Receptors confocal Microscopy	368	(26)
CELIA J. SNYMAN
DESHANDRA M. RAIDOO
KANTI D. BHOOLA
23. Observation of Microcirculatory Kinetics by Real-Time Confocal Laser Scanning Microscopy	394	(31)
KAZUHIRO YAMAGUCHI
Section V. Imaging of Ions	425	(57)
24. Confocal Imaging Analysis of Intracellular Ions in Mixed Cellular Systems or in Situ Using Two Types of Confocal Microscopic Systems	425	(16)
HISAYUKI OHATA
MASAYUKI YAMAMOTO
YOSUKE UJIKE
GOUSEI RIE
KAZUTAKA MOROSE
25. Confocal Ca^(2+) Imaging of Organelles,Cells, Tissues, and Organs	441	(28)
DAVID ALAN WILLIAMS
DAVID N. BOWSER
STEVEN PETROU
26. Using Confocal Microscopy and Fluorescent Indicator, 6-Methoxy-N-ethylquinolinium Iodide, to Measure Changes in Intracellular Chloride	469	(13)
JON R. INGLEFIELD
ROCHELLE D.
SCHWARTZ-BLOOM
Section VI. Imaging of Specialized Tissues	482	(91)
27. Measurement of Mineral Gain and Loss on Dental Enamel and Dentin Using Confocal Microscopy	482	(14)
CARLOS GONZALEZ-CABEZAS
MARGHERITA FONTANA
GEORGE K. STOOKEY
28. Characterization of Ocular Cellular and Extracellular Structures Using Confocal Microscopy and Computerized Three-Dimensional Reconstruction	496	(17)
DANIEL BROTCHIE
NEIL ROBERTS
MIKE BIRCH
PENNY HOGG
C. VYVYAN HOWARD
IAN GRIERSON
29. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo	513	(23)
BARRY R. MASTERS
PETER T. C. SO
KI HEAN KIM
CHRISTOF BUEHLER
ENRICO GRATTON
30. Video-Rate, Scanning Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the Eye	536	(27)
BARRY R. MASTERS
MATTHIAS BOHNKE
31. In Vivo Imaging of Mammalian CNS Neurons with ICON	563	(10)
RALF ENGELMANN
BERNHARD A. SABEL
Section VII. Imaging Viruses and Fungi	573	(52)
32. Identification of Viral Infection by Confocal Microscopy	573	(18)
DAVID N. HOWELL
SARA E. MILLER
33. Using Confocal Microscopy to Study Virus Binding and Entry into Cells	591	(16)
ALAIN VANDERPLASSCHEN
GEOFFREY L. SMITH
34. Fluorescent Labels, Confocal Microscopy, and Quantitative Image Analysis in Study of Fungal Biology	607	(18)
RUSSELL N. SPEAR
DANIEL CULLEN
JOHN H. ANDREWS
AUTHOR INDEX	625	(22)
SUBJECT INDEX	647