Cryopreservation And Freeze-drying Protocols

by Day, John G.; Stacey, Glyn N.

• ISBN13: 9781588293770
• ISBN10: 1588293777
• Edition: 2nd
• Format: Hardcover
• Copyright: 2007-06-20
• Publisher: Humana Pr Inc

Summary

Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995. This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.

Table of Contents

Preface	p. v
Contributors	p. ix
Glossary of Specialized Terms	p. xi
Long-Term Ex Situ Conservation of Biological Resources and the Role of Biological Resource Centers	p. 1
The Principles of Freeze-Drying	p. 15
Principles of Cryopreservation	p. 39
Lyophilization of Proteins	p. 59
Vacuum-Drying and Cryopreservation of Prokaryotes	p. 73
Freeze-Drying of Yeast Cultures	p. 99
Cryopreservation of Yeast Cultures	p. 109
Freeze-Drying Fungi Using a Shelf Freeze-Drier	p. 119
Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying	p. 127
Cryopreservation of Microalgae and Cyanobacteria	p. 141
Cryopreservation of Plant Cell Suspensions	p. 153
Cryopreservation of Shoot Tips and Meristems	p. 163
Cryopreservation of Desiccation-Tolerant Seeds	p. 185
Cryopreservation of Fish Sperm	p. 203
Cryopreservation of Avian Spermatozoa	p. 219
Cryopreservation of Animal and Human Cell Lines	p. 227
Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use	p. 237
Cryopreservation of Human Embryonic Stem Cell Lines	p. 261
Cryopreservation of Primary Animal Cell Cultures	p. 271
Cryopreservation of Red Blood Cells and Platelets	p. 283
Cryopreservation of Mammalian Semen	p. 303
Cryopreservation of Mammalian Oocytes	p. 313
Cryopreservation of Mammalian Embryos	p. 325
Index	p. 341
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