Cryptosporidium

by Smith, M.; Thompson, K. C.; Royal Society of Chemistry (Great Britain)

• ISBN13: 9780854048403
• ISBN10: 0854048405
• Format: Hardcover
• Copyright: 2001-12-01
• Publisher: Royal Society of Chemistry

Summary

Cryptosporidium, in its various forms, is a widely recognized cause of outbreaks of waterborne disease. Regulatory bodies worldwide are increasingly requiring the development of "fit-for-purpose" detection methods for this protozoan parasite, but analysis is often problematic. Bringing together international academic and industry-based experts, this book provides a comprehensive review of the current state of analytical techniques for the detection of cryptosporidium, as well as looking at likely future developments. In particular, the issues of species identification and oocyst viability are addressed. Quality assurance issues and potential problems associated with the new cryptosporidium regulations are also highlighted. The extent of the perceived problems and the regulatory backdrop against which the analysis must be carried out are also discussed. Scientists in the water industry, environmental testing laboratories, researchers, consultants, environmental health professionals, food manufacturers and regulatory or environmental bodies are among the many who should read this book. In addition, anyone with an interest in microbiological challenges and problem-solving will welcome the coverage.

Table of Contents

Cryptosporidium: The Analytical Challenge	1	(43)
H. V. Smith
A. Ronald
Molecular Epidemiology and Systematics of Cryptosporidium parvum	44	(7)
Una M. Morgan
Lihua Xiao
Ronald Fayer
Altaf A. Lal
R. C. Andrew Thompson
Molecular and Phenotypic Analysis of Cryptosporidium parvum Oocysts of Human and Animal Origin	51	(5)
G. Widmer
X. Feng
D. Akiyoshi
S. M. Rich
B. Stein
S. Tzipori
Complying with the New Cryptosporidium Regulations	56	(6)
P. Jiggins
Cryptosporidiosis in Healthy Adult Volunteers	62	(11)
C. L. Chappell
P. C. Okhuysen
Trial of a Method for Continuous Monitoring of the Concentration of Cryptosporidium Oocysts in Treated Drinking Water for Regulatory Purposes	73	(11)
D. P. Casemore
B. Hoyle
P. Tynan
Mark S. Smith
A Dielectrophoresis System for Rapid Analysis of Cryptosporidium parvum	84	(4)
A. P. Brown
W. B. Betts
A Review of Methods for Assessing the Infectivity of Cryptosporidium parvum Using In-vitro Cell Culture	88	(8)
P. A. Rochelle
R. De Leon
Applications of MALDI-TOF Mass Spectrometry in the Analysis of Cryptosporidium	96	(5)
K. Hall
M. A. Claydon
D. J. Evason
M. S. Smith
J. Watkins
Some Observations on Factors which Affect Recovery Efficiency in Cryptosporidium Analysis	101	(10)
A. P. Walker
Development of a Novel Method for the Capture, Recovery and Analysis of Cryptosporidium Oocysts from High Volume Water Samples	111	(9)
A. C. Parton
A. Parton
B. Brewin
K. Bergmann
E. Hewson
D. Sartory
The Experience of the Leap Proficiency Scheme With Respect to Cryptosporidium Testing	120	(13)
K. Clive Thompson
Barry May
Diane Corscadden
John Watkins
An Evaluation of the Current Methods for the Detection and Enumeration of Cryptosporidium in Water	133	(10)
Carol Francis
Diane Corscadden
John Watkins
Mark Wyer
Automated Detection and Viability Assessment	143	(12)
D. A. Veal
M. R. Dorsch
B. C. Ferrari
Can We Believe Our Results?	155	(7)
Frank W. Schaefer
Subject Index	162